The effect of leaf hydroalcoholic extract of Ephedra pachyclada infertility in male rats treated with cyclophosphamide: An experimental study.

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY

International Journal of Reproductive Biomedicine Pub Date : 2023-05-01 DOI:10.18502/ijrm.v21i4.13268

Shiva Saleh, Aref Ghanaatpisheh, Hoda Haghshenas, Negar Parvin, Elmira Mikaeiliagah, Hossein Kargar Jahromi, Bahare Ebrahimi

{"title":"The effect of leaf hydroalcoholic extract of Ephedra pachyclada infertility in male rats treated with cyclophosphamide: An experimental study.","authors":"Shiva Saleh, Aref Ghanaatpisheh, Hoda Haghshenas, Negar Parvin, Elmira Mikaeiliagah, Hossein Kargar Jahromi, Bahare Ebrahimi","doi":"10.18502/ijrm.v21i4.13268","DOIUrl":null,"url":null,"abstract":"Background: Cyclophosphamide (CP) has clinical applications in treating diverse malignancies and autoimmune disorders; at the same time, it also has harmful effects on the body tissues, particularly the genitals. The most significant side effects of CP are changing the reproductive system's function and infertility.Objective: This study determines the Ephedra hydroalcoholic extract (EP) role on testicular tissue and the pituitary-gonadal axis in CP-treated male rats.Materials and methods: In this experimental study, 48 adult Wistar rats were separated into 6 groups (n = 8/each): control, sham, CP recipients, and CP recipients with gavage-fed EP (250, 500, and 1000 mg/kg). On the 29<math><msup><mrow></mrow><mtext>th</mtext></msup></math> day, the blood of the weighed animals' was drawn from their heart, and serum concentrations of follicle-stimulating hormone, luteinizing hormone, and testosterone were measured. After preparing testicular tissue segments, cells were counted.Results: While CP decreased follicle-stimulating hormone, luteinizing hormone, and testosterone levels (p <math><mo><</mo></math> 0.05), the use of EP changed them and even reached the control. Serum gonadotropin-releasing hormone increased significantly in all EP groups compared to the control and CP groups. Compared to the control, a significant decrease in total antioxidant capacity and plasma glutathione peroxidase was observed in the CP groups. EP (all doses) significantly increased their concentration compared to the CP group (p <math><mo><</mo></math> 0.05); significant reduction in serum total oxidant status and malondialdehyde in CP groups changed by EP (p <math><mo><</mo></math> 0.05). Although CP's role on spermatogonia counts (57.5 <math><mo>±</mo></math> 5.2 in CP, 67.1 <math><mo>±</mo></math> 6.0 in control), higher doses of EP had no significant effect on this but did affect spermatocyte and spermatid cells count.Conclusion: Due to its antioxidant characteristics, EP mitigated the effects of CP on the investigated parameters in rats.","PeriodicalId":14386,"journal":{"name":"International Journal of Reproductive Biomedicine","volume":"21 4","pages":"285-294"},"PeriodicalIF":1.6000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227357/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Reproductive Biomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijrm.v21i4.13268","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Cyclophosphamide (CP) has clinical applications in treating diverse malignancies and autoimmune disorders; at the same time, it also has harmful effects on the body tissues, particularly the genitals. The most significant side effects of CP are changing the reproductive system's function and infertility.

Objective: This study determines the Ephedra hydroalcoholic extract (EP) role on testicular tissue and the pituitary-gonadal axis in CP-treated male rats.

Materials and methods: In this experimental study, 48 adult Wistar rats were separated into 6 groups (n = 8/each): control, sham, CP recipients, and CP recipients with gavage-fed EP (250, 500, and 1000 mg/kg). On the 29 $^{th}$ day, the blood of the weighed animals' was drawn from their heart, and serum concentrations of follicle-stimulating hormone, luteinizing hormone, and testosterone were measured. After preparing testicular tissue segments, cells were counted.

Results: While CP decreased follicle-stimulating hormone, luteinizing hormone, and testosterone levels (p $<$ 0.05), the use of EP changed them and even reached the control. Serum gonadotropin-releasing hormone increased significantly in all EP groups compared to the control and CP groups. Compared to the control, a significant decrease in total antioxidant capacity and plasma glutathione peroxidase was observed in the CP groups. EP (all doses) significantly increased their concentration compared to the CP group (p $<$ 0.05); significant reduction in serum total oxidant status and malondialdehyde in CP groups changed by EP (p $<$ 0.05). Although CP's role on spermatogonia counts (57.5 $\pm$ 5.2 in CP, 67.1 $\pm$ 6.0 in control), higher doses of EP had no significant effect on this but did affect spermatocyte and spermatid cells count.

Conclusion: Due to its antioxidant characteristics, EP mitigated the effects of CP on the investigated parameters in rats.

Abstract Image

查看原文本刊更多论文

麻黄叶水醇提取物对环磷酰胺治疗雄性不育大鼠影响的实验研究。

背景:环磷酰胺(CP)在治疗多种恶性肿瘤和自身免疫性疾病中有临床应用;同时，它对身体组织，特别是生殖器也有有害的影响。CP最显著的副作用是改变生殖系统功能和不育。目的:研究麻黄水酒精提取物(EP)对cp治疗的雄性大鼠睾丸组织和垂体-性腺轴的作用。材料与方法:将48只成年Wistar大鼠分为6组(n = 8/ kg):对照组、假手术组、CP组、CP组灌胃EP(250、500、1000 mg/kg)。第29天，称重动物取心脏血，测定血清促卵泡激素、促黄体生成素和睾酮浓度。制备睾丸组织段后，进行细胞计数。结果:CP降低了促卵泡激素、黄体生成素和睾酮水平(p < 0.05)，而EP则改变了促卵泡激素、黄体生成素和睾酮水平，甚至达到了对照组。与对照组和CP组相比，EP组血清促性腺激素释放激素显著升高。与对照组相比，CP组总抗氧化能力和血浆谷胱甘肽过氧化物酶显著降低。与CP组相比，EP(所有剂量)显著提高了其浓度(p 0.05);EP改变了CP组血清总氧化状态和丙二醛的显著降低(p 0.05)。尽管CP对精原细胞计数有影响(CP组为57.5±5.2，对照组为67.1±6.0)，但高剂量EP对其没有显著影响，但确实影响精母细胞和精母细胞计数。结论:EP因其抗氧化特性，减轻了CP对大鼠各项指标的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Reproductive Biomedicine OBSTETRICS & GYNECOLOGY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

16 weeks

期刊介绍： The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.