Neuroprotective effect of Prunus avium on streptozotocin induced neurotoxicity in mice

Edula Vinitha, Hanish J.C. Singh, Rahul Motiram Kakalij, Rahul Padmakar Kshirsagar, Boyina Hemanth Kumar, Prakash V. Diwan
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引用次数: 10

Abstract

Objective

To evaluate the anti-amnesic and neuroprotective activity of ethanolic extract of Prunus avium (EEPA) on streptozotocin (STZ) induced neurotoxicity in mice.

Methods

The mice were pre-treated with EEPA at selective doses (200, 400 mg/kg, p.o.) for a period of 3 weeks followed by intracerebroventricular injection (ICV) of STZ (0.5 mg/kg). Neurobehavioral-alterations were evaluated using Y-maze and elevated plus maze. Biochemical markers, such as acetylcholinesterase (AChE), corticosterone, thiobarbituric reactive substances (TBARS), tissue nitrite, antioxidants, such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase were estimated.

Results

Obtain results revealed those 28 days of treatment with EEPA was effective in averting neurotoxicity. EEPA supplementation significantly reduced AChE, corticosterone, TBARS, tissue nitrite levels and ameliorated the deficits in learning and memory impairment with increased levels of antioxidants.

Conclusions

These results envisage that ethanolic extract of Prunus avium exhibit cognitive improvement which is most likely related, at least in part, to its antioxidant and neuroprotective activity. Further studies are suggested to evaluate the isolated bioactive Prunus avium fruits to identify the molecular mechanism involved in modulation of cholinergic transmission.

鸟李对链脲佐菌素所致小鼠神经毒性的保护作用
目的观察李醇提物(EEPA)对链脲佐菌素(STZ)致小鼠神经毒性的抗遗忘作用和神经保护作用。方法采用选择性剂量EEPA(200、400 mg/kg, p.o)预处理小鼠,连续3周后再脑室内注射STZ (0.5 mg/kg)。采用y型迷宫和高架正迷宫评估神经行为改变。生化指标,如乙酰胆碱酯酶(AChE)、皮质酮、硫代巴比妥反应性物质(TBARS)、组织亚硝,抗氧化剂,如超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)和过氧化氢酶。结果EEPA治疗28 d可有效避免神经毒性。补充EEPA可显著降低乙酰胆碱酯酶、皮质酮、TBARS和组织亚硝酸盐水平,并通过增加抗氧化剂水平改善学习和记忆障碍的缺陷。结论桃李醇提物具有明显的认知改善作用,这可能与其抗氧化和神经保护作用有关。研究人员建议进一步对具有生物活性的李果实进行鉴定,以确定其调控胆碱能传递的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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