Phytochemical screening and antibacterial assessment of two macroalgae Ulva papenfussi and Ulva nematoidea (Chlorophyta) against the bacterium Vibrio parahaemolyticus.

IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED
Food Science and Technology International Pub Date : 2024-06-01 Epub Date: 2023-03-27 DOI:10.1177/10820132231165540
Jessie Vargas Cárdenas, Jorge Chávez Pérez, Nancy Martínez Ordinola, Ivan Soto Rodríguez, Luis Otavio Brito, Silvio Ricardo Maurano Peixoto, Alfredo Olivera Galvez
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引用次数: 0

Abstract

This study was conducted to determine whether the macroalgae Ulva papenfussi and Ulva nematoidea could be alternatives for preventing Litopenaeus vannamei vibriosis caused by the bacterium Vibrio parahaemolyticus. Phytochemical screening was performed on methanolic extracts to qualitatively determine the main groups of bioactive compounds, previous to an in vitro antibacterial test against V. parahaemolitycus. Phenols, polyphenols, flavonoids, and the high presence of carbohydrates were found in both macroalgae. U. papenfussi showed more presence of lipids and alkaloids than U. nematoidea. Macroalgae extracts prepared (v:v) with a 1:1 methanol: dichloromethane solvent was used for the in vitro test using the disc diffusion method (MDD). Filter paper discs impregnated with 1.0, 1.5, 2.0, 3.0, and 4.0 mg of the extracts showed antibacterial activity against V. Parahaemolitycus in a dose-dependent manner in both macroalgae. The inhibition zone varied significantly (p < 0.05) from 8.33 ± 0.12 to 11.41 ± 0.73 mm for 1 to 3 mg of extract levels, respectively. In conclusion, both macroalgae have antibacterial activity in their crude extracts against this bacteria. It is suggested to evaluate it as a feed additive for L. vannamei. This study is the first report on a phytochemical screening and antibacterial activity of these macroalgae against V. parahaemolyticus.

针对副溶血性弧菌的两种大型藻类(叶绿藻)莼菜(Ulva papenfussi)和石莼(Ulva nematoidea)的植物化学筛选和抗菌评估
本研究旨在确定大型藻类石莼和针莼是否可作为预防副溶血性弧菌引起的万年青弧菌病的替代品。在对副溶血弧菌进行体外抗菌试验之前,对甲醇提取物进行了植物化学筛选,以确定生物活性化合物的主要类别。在两种大型藻类中都发现了酚类、多酚类、类黄酮以及大量碳水化合物。U. papenfussi 的脂类和生物碱含量高于 U. nematoidea。用 1:1 甲醇:二氯甲烷溶剂(v:v)制备的大型藻类提取物被用于体外试验,采用的是圆片扩散法(MDD)。浸渍了 1.0、1.5、2.0、3.0 和 4.0 毫克提取物的滤纸圆盘显示,两种大型藻类对副伞花叶病毒都具有抗菌活性,且呈剂量依赖性。抑制区的变化很大(p. vannamei)。本研究首次报道了这些大型藻类的植物化学筛选和对副溶血弧菌的抗菌活性。
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来源期刊
Food Science and Technology International
Food Science and Technology International 工程技术-食品科技
CiteScore
5.80
自引率
4.30%
发文量
63
审稿时长
18-36 weeks
期刊介绍: Food Science and Technology International (FSTI) shares knowledge from leading researchers of food science and technology. Covers food processing and engineering, food safety and preservation, food biotechnology, and physical, chemical and sensory properties of foods. This journal is a member of the Committee on Publication Ethics (COPE).
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