The effect of encomir-93 mimic transfection on the expression of miR-93 and PSA and androgen receptor in prostate cancer LNcap cell line.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-31 eCollection Date: 2023-09-01 DOI:10.1515/hmbci-2022-0096

Hossein Mokhtari, Ali Ebrahimi, Mohaddeseh Nejati, Zeinab Barartabar, Mehdi Damchi, Abbas Khonakdar-Tarsi, Mahlagha Zahedi

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引用次数: 0

Abstract

Objectives: Prostate cancer (PCa) is one of the most common cancers in men with high mortality rate which is a major concern for men's health. However, the molecular mechanisms remain poorly understood. miR-93 is an important oncogene which may have important function in prostate cancer.So, this study aimed to predict that encomir-93 mimic transfection on the expression of miR-93 and PSA and AR in prostate cancer LNcap cell line.

Methods: Lymph node carcinoma of the prostate (LNCaP) was cultured and then miR-93 mimics was designed, synthesized and the transfected to LNCaP. The expression level of prostate-specific antigen (PSA) and androgen receptor (AR) was determined via Real-time PCR after treated with 15 pmol of miR-93 mimics.

Results: miR-93 mimic transfection led to significant increase in PSA and AR expression in comparison with control group (p≤0.05).

Conclusions: The miR-93 and its target genes has important role in PCa progression via enhancement in PSA and AR expression. Further research on the function of the miR-93 and its target genes in tumorgenesis and progression PCa could be helpful for the treatment of prostate cancer.

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encomir-93模拟转染对前列腺癌症LNcap细胞系中miR-93、PSA和雄激素受体表达的影响。

目的：癌症（PCa）是男性最常见的癌症之一，死亡率高，是男性健康的主要问题。然而，对其分子机制仍知之甚少。miR-93是一种重要的癌基因，可能在癌症中发挥重要作用。因此，本研究旨在预测encomir-93模拟转染对前列腺癌症LNcap细胞系中miR-93、PSA和AR表达的影响。方法：培养前列腺淋巴结癌（LNCaP），设计、合成miR-93模拟物并转染LNCaP。用15pmol的miR-93模拟物处理后，通过实时PCR测定前列腺特异性抗原（PSA）和雄激素受体（AR）的表达水平。结果：与对照组相比，miR-93模拟转染导致PSA和AR表达显著增加（p≤0.05）。结论：miR-93及其靶基因通过增强PSA和AR的表达，在前列腺癌的进展中发挥重要作用。进一步研究miR-93及其靶基因在肿瘤发生和进展PCa中的作用可能有助于癌症的治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.