The possible ameliorative role of Lycopene on Tributyltin induced thyroid damage in adult male albino rats (histological, immunohistochemical and biochemical study).

IF 1.1 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2023-07-04 Epub Date: 2023-05-01 DOI:10.1080/01913123.2023.2205922

Ghada A Elsammak, Aliaa Talaat, Samar Reda

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引用次数: 0

Abstract

Tributyltin is used in industrial applications. This current research aimed to study the effect of Tributyltin on the thyroid gland structure and function of adult male albino rats and the protective effect of Lycopene. Twenty-one male adult albino rats were classified into three groups: Control, treated that received Tributyltin, and protective that received Lycopene with Tributyltin. At the end of the experiment, blood samples were collected and T4, T3, and (TSH) were measured. Tissue superoxide dismutase (SOD) and malondialdehyde (MDA) were estimated. Thyroid gland specimens were processed for histological and immunohistochemical examination. Then morphometric and statistical analyses were done. The treated group showed affection in thyroid function and histological structure as vacuolated colloid and cytoplasm and dark nuclei. Ultrastructurally, follicular cells showed irregular shrunken nuclei, atrophied apical microvilli, vacuoles, multiple lysosomal granules, mitochondria with destructed cristae, and extensively dilated rough endoplasmic reticulum. There was increase in Caspase-3 immunoexpression and decrease in Beclin-1 immunoexpression. The thyroid structure and biochemical markers improved after Lycopene administration. The thyroid gland damage caused by Tributyltin is ameliorated by Lycopene.

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番茄红素对三丁基锡诱导的成年雄性白化大鼠甲状腺损伤的可能改善作用（组织学、免疫组织化学和生物化学研究）。

三丁基锡用于工业用途。本研究旨在探讨三丁基锡对成年雄性白化大鼠甲状腺结构和功能的影响以及番茄红素的保护作用。21 只成年雄性白化大鼠被分为三组：对照组、接受三丁基锡治疗组和接受番茄红素与三丁基锡联合保护组。实验结束时，收集血液样本并测量 T4、T3 和（促甲状腺激素）。对组织超氧化物歧化酶（SOD）和丙二醛（MDA）进行估算。对甲状腺标本进行组织学和免疫组化检查。然后进行形态计量学和统计学分析。治疗组的甲状腺功能和组织学结构表现为胶质和细胞质空泡化，细胞核变暗。超微结构显示，滤泡细胞核不规则萎缩，顶端微绒毛萎缩，有空泡，溶酶体颗粒多，线粒体嵴破坏，粗面内质网广泛扩张。Caspase-3免疫表达增加，Beclin-1免疫表达减少。服用番茄红素后，甲状腺结构和生化指标均有所改善。番茄红素可改善三丁基锡对甲状腺的损伤。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.