A custom-made weight-drop impactor to produce consistent spinal cord injury outcomes in a rat model.

IF 1.8 4区医学 Q4 NEUROSCIENCES

Translational Neuroscience Pub Date : 2023-01-01 DOI:10.1515/tnsci-2022-0287

Ali Jarragh, Ali Shuaib, Ghanim Al-Khaledi, Fatima Alotaibi, Sulaiman Al-Sabah, Willias Masocha

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引用次数: 0

Abstract

Objective: The main objective of this study is to design a custom-made weight-drop impactor device to produce a consistent spinal cord contusion model in rats in order to examine the efficacy of potential therapies for post-traumatic spinal cord injuries (SCIs).

Methods: Adult female Sprague-Dawley rats (n = 24, 11 weeks old) were randomly divided equally into two groups: sham and injured. The consistent injury pattern was produced by a 10 g stainless steel rod dropped from a height of 30 mm to cause (0.75 mm) intended displacement to the dorsal surface of spinal cord. The neurological functional outcomes were assessed at different time intervals using the following standardized neurobehavioral tests: Basso, Beattie, and Bresnahan (BBB) scores, BBB open-field locomotion test, Louisville Swim Scale (LSS), and CatWalk gait analysis system.

Results: Hind limb functional parameters between the two groups using BBB scores and LSS were significantly different (p < 0.05). There were significant differences (p < 0.05) between the SCI group and the sham group for the hind limb functional parameters using the CatWalk gait analysis.

Conclusion: We developed an inexpensive custom-made SCI device that yields a precise adjustment of the height and displacement of the impact relative to the spinal cord surface.

Abstract Image

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在大鼠模型中，定制的重量下降撞击器产生一致的脊髓损伤结果。

目的:本研究的主要目的是设计一种定制的减重撞击装置，以产生一致的大鼠脊髓挫伤模型，以研究创伤后脊髓损伤(SCIs)的潜在治疗方法的疗效。方法:成年雌性Sprague-Dawley大鼠24只，11周龄，随机分为假手术组和损伤组。将一根10克不锈钢棒从30毫米的高度落下，造成脊髓背表面0.75毫米的预定位移，从而产生一致的损伤模式。采用以下标准化神经行为测试:Basso, Beattie, and Bresnahan (BBB)评分、BBB露天运动测试、Louisville游泳量表(LSS)和CatWalk步态分析系统，在不同的时间间隔评估神经功能结果。结果:两组用BBB评分和LSS评价后肢功能参数差异有统计学意义(p < 0.05)。采用CatWalk步态分析，脊髓损伤组与假手术组的后肢功能参数差异有统计学意义(p < 0.05)。结论:我们开发了一种廉价的定制SCI装置，可以精确调节撞击高度和相对于脊髓表面的位移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Neuroscience NEUROSCIENCES-

CiteScore

3.00

自引率

4.80%

发文量

审稿时长

>12 weeks

期刊介绍： Translational Neuroscience provides a closer interaction between basic and clinical neuroscientists to expand understanding of brain structure, function and disease, and translate this knowledge into clinical applications and novel therapies of nervous system disorders.