Effect of irinotecan administration on amiloride-sensitive sodium taste responses in mice

IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Nami Obayashi, Nobuyuki Sakayori, Hiroyuki Kawaguchi, Makoto Sugita
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引用次数: 1

Abstract

Taste alteration is a frequently reported side effect in patients receiving the chemotherapeutic agent, irinotecan. However, the way in which irinotecan causes taste disturbance and the type of taste impairment that is affected remain elusive. Here, we used the two-bottle preference test to characterize behavioral taste responses and employed immunohistochemical analyses to clarify the types and mechanisms of taste alteration induced, in mice, by irinotecan administration. Irinotecan administration resulted in a reduced intake of sodium taste solution but had no effect on sweet taste responses, as determined in the two-bottle preference test. In the presence of amiloride, which inhibits the function of the epithelial sodium channel (ENaC) in the periphery, the intake of sodium taste solution was comparable between the irinotecan-treated and control groups. Immunohistochemical analyses revealed that α-ENaC immunoreactivity detected in taste bud cells decreased slowly after irinotecan administration, and that administration of irinotecan had little effect on the number of cells expressing the cellular proliferation marker, Ki67, within or around taste buds. Our results imply that irinotecan administration may be responsible for altered behavioral sodium taste responses originating from ENaC function in the periphery, while being accompanied by the reduction of α-ENaC expression at the apical membrane of taste receptor cells without disturbing taste cell renewal.

伊立替康给药对小鼠阿米洛利钠敏感味觉反应的影响
味觉改变是接受伊立替康化疗的患者经常报道的副作用。然而,伊立替康引起味觉障碍的方式和受影响的味觉损伤类型仍然难以捉摸。在这里,我们使用两瓶偏好测试来表征行为味觉反应,并使用免疫组织化学分析来阐明伊立替康在小鼠中诱导的味觉改变的类型和机制。伊立替康的使用减少了钠味溶液的摄入量,但对甜味反应没有影响,正如两瓶偏好测试所确定的那样。阿米洛利能抑制外周上皮钠通道(ENaC)的功能,在阿米洛利存在的情况下,伊立替康治疗组和对照组的钠盐溶液摄入量相当。免疫组化分析显示,伊立替康给药后,味蕾细胞α-ENaC免疫反应性缓慢下降,且伊立替康对味蕾内和味蕾周围表达细胞增殖标志物Ki67的细胞数量影响不大。我们的研究结果表明,伊立替康可能改变了外周ENaC功能引起的行为钠味觉反应,同时伴随着味觉受体细胞顶膜α-ENaC表达的减少,但不影响味觉细胞的更新。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
European Journal of Oral Sciences
European Journal of Oral Sciences 医学-牙科与口腔外科
CiteScore
3.50
自引率
5.30%
发文量
61
审稿时长
2 months
期刊介绍: The European Journal of Oral Sciences is an international journal which publishes original research papers within clinical dentistry, on all basic science aspects of structure, chemistry, developmental biology, physiology and pathology of relevant tissues, as well as on microbiology, biomaterials and the behavioural sciences as they relate to dentistry. In general, analytical studies are preferred to descriptive ones. Reviews, Short Communications and Letters to the Editor will also be considered for publication. The journal is published bimonthly.
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