Effect of three clinical therapies on cytokines modulation in the hip articular cartilage and bone improvement in rat early osteonecrosis of the femoral head

IF 4.1 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomedical Journal Pub Date : 2023-12-01 DOI:10.1016/j.bj.2022.11.004

Shan-Ling Hsu , Shun-Wun Jhan , Chieh-Cheng Hsu , Yi-No Wu , Kay L.H. Wu , Chun-En Aurea Kuo , Hung-Wen Chiu , Jai-Hong Cheng

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引用次数: 1

Abstract

Background

Extracorporeal shockwave therapy (ESWT) and adipose-derived mesenchymal stem cells (ADSCs) have been used clinically for the treatment of osteonecrosis of the femoral head (ONFH). The study elucidated that ESWT, ADSCs, and combination therapy modulated pro-inflammatory cytokines in the articular cartilage and subchondral bone of early rat ONFH.

Methods

ESWT and ADSCs were prepared and isolated for treatment. Micro-CT, pathological analysis, and immunohistochemistry were performed and analysed.

Results

After treatments, subchondral bone of ONFH was improved in trabecular bone volume (BV/TV) (p < 0.001), thickness (Tb.Th) (p < 0.01 and 0.001), and separation (Tb.Sp) (p < 0.001) and bone mineral density (BMD) (p < 0.001) using micro-CT analysis. The articular cartilage was protected and decreased apoptosis markers after all the treatments. The expression of IL33 (p < 0.001), IL5 (p < 0.001), IL6 (p < 0.001), and IL17A (p < 0.01) was significantly decreased in the ESWT, ADSCs, and Combination groups as compared with ONFH group. The IL33 receptor ST2 was significantly increased after treatment (p < 0.001) as compared with ONFH group. The Combination group (p < 0.01) decreased the expression of IL6 better than the ESWT and ADSCs groups.

Conclusion

ESWT, ADSCs and combination therapy significantly protected articular cartilage and subchondral bone of early rat ONFH by modulating the expression of pro-inflammatory cytokines including, IL33 and its receptor ST2, IL5, IL6, and IL17A.

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三种临床治疗方法对大鼠股骨头早期骨坏死髋关节软骨细胞因子调节及骨改善的影响

体外冲击波疗法(ESWT)和脂肪源性间充质干细胞(ADSCs)已被临床用于治疗股骨头骨坏死(ONFH)。研究表明，ESWT、ADSCs和联合治疗可调节早期ONFH大鼠关节软骨和软骨下骨的促炎细胞因子。方法制备seswt和ADSCs进行分离处理。显微ct、病理分析、免疫组织化学分析。结果治疗后ONFH软骨下骨的骨小梁体积(BV/TV)明显改善(p <0.001)，厚度(Tb.Th) (p <0.01和0.001)，分离度(Tb.Sp) (p <0.001)和骨密度(BMD) (p <0.001)。各处理后关节软骨均受到保护，凋亡标志物减少。IL33的表达(p <0.001)， IL5 (p <0.001)， il - 6 (p <0.001)， IL17A (p <与ONFH组相比，ESWT组、ADSCs组和联合组的细胞凋亡均显著降低。治疗后il - 33受体ST2明显升高(p <0.001)，与ONFH组比较。联合组(p <0.01)降低il - 6表达的效果优于ESWT和ADSCs组。结论eswt、ADSCs及联合治疗通过调节il - 33及其受体ST2、il - 5、il - 6、il - 17a等促炎因子的表达，对早期ONFH大鼠关节软骨和软骨下骨有明显的保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Journal Medicine-General Medicine

CiteScore

11.60

自引率

1.80%

发文量

128

审稿时长

42 days

期刊介绍： Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs. Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology. A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.