Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets

IF 6.3
Fengjie Ji , Huansheng Yang , Qiye Wang , Jianzhong Li , Hanlin Zhou , Shengmin Liu
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引用次数: 1

Abstract

Antibiotic resistance of pathogens, which is caused by the abuse of in-feed antibiotics, threatens the sustainable development of livestock production. The present study aimed to investigate the efficiency of porcine intestinal antimicrobial peptide (PIAP) as an alternative to in-feed antibiotics in terms of growth performance, intestinal morphology, digestive enzymes and immunity, and microbiota community of the post-weaning piglets. A total of 204 piglets (Duroc × Landrace × Yorkshire, weaned at 28 d age) with a similar body weight of 7.97 ± 1.04 kg were randomly allocated to 4 groups (51 piglets per group): (1) control group: basal diet; (2) AB group: antibiotic, basal diet + chlortetracycline (1000 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37); (3) P1 group: basal diet + a relatively low dose of PIAP (400 mg/kg from d 1 to 24; 300 mg/kg from d 25 to 37); (4) P2 group, basal diet + a relatively high dose of PIAP (600 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37). The results showed that serum indicators of hepatocyte damage and relative organ weight were not affected by these treatments (P > 0.05). Compared with the AB treatment, the P1 treatment remarkably decreased jejunal crypt depth and increased jejunal and ileal villus height:crypt depth ratio (P < 0.05). The values of jejunal maltase, lactase, sucrase, intestinal alkaline phosphatase, and secretory immunoglobulin A (SIgA) in the P1 group were sharply increased compared with those in the control and P2 groups (P < 0.05). Compared with the control group, the P1 group decreased serum concentrations of D-lactate, diamine oxidase, and endotoxin (P < 0.05), and increased the abundance of Lactobacillus reuteri (P < 0.05) in the colonic feces. Furthermore, there was a positive correlation between the abundance of L. reuteri and the concentrations of maltase, lactase, sucrase, and SIgA (P < 0.05). Collectively, dietary supplementation with a relatively low dose of PIAP (400 mg/kg from d 1 to 24; 300 mg/kg from d 25 to 37) demonstrates beneficial effects on intestinal morphology, digestive enzymes, immunity, and permeability by shaping the gut microbiota composition in weaned piglets. This study will provide a valuable reference for using PIAP as an in-feed antibiotic alternative in swine production.

Abstract Image

猪肠道抗菌肽作为饲料中的抗生素替代品,通过塑造断奶仔猪的肠道微生物群来改善肠道消化和免疫力
饲料中滥用抗生素引起的病原体的抗生素耐药性威胁着畜牧业的可持续发展。本研究旨在研究猪肠道抗菌肽(PIAP)作为饲料中抗生素的替代品在断奶后仔猪的生长性能、肠道形态、消化酶和免疫力以及微生物群群落方面的有效性。将204头体重相似的仔猪(杜洛克×长白×约克郡,28日龄断奶)随机分为4组(每组51头):(1)对照组:基础日粮;(2) AB组:抗生素,基础日粮+金霉素(第1-24天1000mg/kg;第25-37天500mg/kg);(3) P1组:基础日粮+相对低剂量的PIAP(第1-24天400 mg/kg;第25-37天300 mg/kg);(4) P2组,基础日粮+相对高剂量的PIAP(第1-24天600 mg/kg;第25-37天500 mg/kg)。结果表明,这些处理对肝细胞损伤的血清指标和相对器官重量没有影响(P>;0.05)。与AB处理相比,P1处理显著降低了空肠隐窝深度,增加了空肠和回肠绒毛高度:隐窝深度比(P<;0.05),与对照组和P2组相比,P1组的分泌性免疫球蛋白A(SIgA)显著增加(P<;0.05)。此外,路氏乳杆菌的丰度与麦芽糖酶、乳糖酶、蔗糖酶和SIgA的浓度呈正相关(P<;0.05)。总的来说,在饮食中补充相对低剂量的PIAP(第1-24天400 mg/kg;第25-37天300 mg/kg)对肠道形态、消化酶、免疫力、,以及通过塑造断奶仔猪的肠道微生物群组成来提高渗透性。本研究将为PIAP作为饲料中抗生素的替代品在猪生产中的应用提供有价值的参考。
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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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