Desulfatitalea alkaliphila sp. nov., an alkalipilic sulfate- and arsenate- reducing bacterium isolated from a terrestrial mud volcano.

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
M A Khomyakova, A Yu Merkel, V S Segliuk, A I Slobodkin
{"title":"Desulfatitalea alkaliphila sp. nov., an alkalipilic sulfate- and arsenate- reducing bacterium isolated from a terrestrial mud volcano.","authors":"M A Khomyakova,&nbsp;A Yu Merkel,&nbsp;V S Segliuk,&nbsp;A I Slobodkin","doi":"10.1007/s00792-023-01297-0","DOIUrl":null,"url":null,"abstract":"<p><p>A novel alkaliphilic sulfate-reducing bacterium, strain M08but<sup>T</sup>, was isolated from a salsa lake of terrestrial mud volcano (Taman Peninsula, Russia). Cells were rod-shaped, motile and Gram-stain-negative. The temperature range for growth was 15-42 °C (optimum at 30 °C). The pH range for growth was 7.0-11.0, with an optimum at pH 8.5-9.0 Strain M08but<sup>T</sup> used sulfate, thiosulfate, sulfite, dimethyl sulfoxide and arsenate as electron acceptors. Acetate, formate, butyrate, fumarate, succinate, glycerol and pyruvate were utilized as electron donors with sulfate. Fermentative growth was observed with fumarate, pyruvate, crotonate. Strain M08but<sup>T</sup> grew chemolithoautotrophically with H<sub>2</sub> and CO<sub>2</sub>. The G + C content of the genomic DNA was 60.1%. The fatty acid profile of strain M08but<sup>T</sup> was characterized by the presence of anteiso-C<sub>15:0</sub> as the major component (68.8%). The closest phylogenetic relative of strain M08but<sup>T</sup> was Desulfatitalea tepidiphila (the order Desulfobacterales) with 96.3% 16S rRNA gene sequence similarity. Based on the phenotypic, genotypic and phylogenetic characteristics of the isolate, strain M08but<sup>T</sup> is considered to represent a novel species of the genus Desulfatitalea, with proposed name Desulfatitalea alkaliphila sp. nov. The type strain of Desulfatitalea alkaliphila is M08but<sup>T</sup> (= KCTC 25382<sup>T</sup> = VKM B-3560<sup>T</sup> = DSM 113909<sup>T</sup> = JCM 39202<sup>T</sup> = UQM 41473<sup>T</sup>).</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":"27 2","pages":"12"},"PeriodicalIF":2.6000,"publicationDate":"2023-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Extremophiles","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00792-023-01297-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

A novel alkaliphilic sulfate-reducing bacterium, strain M08butT, was isolated from a salsa lake of terrestrial mud volcano (Taman Peninsula, Russia). Cells were rod-shaped, motile and Gram-stain-negative. The temperature range for growth was 15-42 °C (optimum at 30 °C). The pH range for growth was 7.0-11.0, with an optimum at pH 8.5-9.0 Strain M08butT used sulfate, thiosulfate, sulfite, dimethyl sulfoxide and arsenate as electron acceptors. Acetate, formate, butyrate, fumarate, succinate, glycerol and pyruvate were utilized as electron donors with sulfate. Fermentative growth was observed with fumarate, pyruvate, crotonate. Strain M08butT grew chemolithoautotrophically with H2 and CO2. The G + C content of the genomic DNA was 60.1%. The fatty acid profile of strain M08butT was characterized by the presence of anteiso-C15:0 as the major component (68.8%). The closest phylogenetic relative of strain M08butT was Desulfatitalea tepidiphila (the order Desulfobacterales) with 96.3% 16S rRNA gene sequence similarity. Based on the phenotypic, genotypic and phylogenetic characteristics of the isolate, strain M08butT is considered to represent a novel species of the genus Desulfatitalea, with proposed name Desulfatitalea alkaliphila sp. nov. The type strain of Desulfatitalea alkaliphila is M08butT (= KCTC 25382T = VKM B-3560T = DSM 113909T = JCM 39202T = UQM 41473T).

Abstract Image

一种从陆相泥火山中分离出来的碱性硫酸盐和砷酸盐还原细菌。
从俄罗斯塔曼半岛陆相泥火山萨尔萨湖中分离到一株新的亲碱硫酸盐还原菌M08butT。细胞呈杆状,可运动,革兰氏染色阴性。生长温度范围为15-42℃(最适温度为30℃)。菌株m08的生长pH范围为7.0 ~ 11.0,最佳生长pH为8.5 ~ 9.0。菌株m08采用硫酸盐、硫代硫酸盐、亚硫酸盐、二甲基亚砜和砷酸盐作为电子受体。以乙酸酯、甲酸酯、丁酸酯、富马酸酯、琥珀酸酯、甘油和丙酮酸酯为电子给体。用富马酸、丙酮酸、巴豆酸观察发酵生长。菌株M08butT在H2和CO2条件下进行了趋化岩石自养生长。基因组DNA中G + C含量为60.1%。菌株M08butT的脂肪酸谱以anteiso-C15:0为主要成分(68.8%)。菌株M08butT的亲缘关系最近的是Desulfatitalea tepidiphila (Desulfatitalea tepidiphila),其16S rRNA基因序列相似性为96.3%。根据分离株的表型、基因型和系统发育特征,认为菌株M08butT代表了Desulfatitalea属的一个新种,建议将菌株命名为Desulfatitalea alkaliphila sp. 11 .该菌株的类型为M08butT (= KCTC 25382T = VKM B-3560T = DSM 113909T = JCM 39202T = UQM 41473T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Extremophiles
Extremophiles 生物-生化与分子生物学
CiteScore
6.80
自引率
6.90%
发文量
28
审稿时长
2 months
期刊介绍: Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信