Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids.

IF 6.7 1区 工程技术 Q1 CELL & TISSUE ENGINEERING
Journal of Tissue Engineering Pub Date : 2023-05-16 eCollection Date: 2023-01-01 DOI:10.1177/20417314231169375
Elloise Z Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H El-Sagheer, Tom Brown, Antonios G Kanaras, Jonathan J West, Ben D MacArthur, Patrick S Stumpf, Richard Oc Oreffo
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引用次数: 0

Abstract

There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to isolate SSCs are restricted by the lack of a defined marker, limiting understanding of SSC fate, immunophenotype, function and clinical application. The current study applied single-cell RNA-sequencing to profile human adult bone marrow populations from 11 donors and identified novel targets for SSC enrichment. Spherical nucleic acids were used to detect these mRNA targets in SSCs. This methodology was able to rapidly isolate potential SSCs found at a frequency of <1 in 1,000,000 in human bone marrow, with the capacity for tri-lineage differentiation in vitro and ectopic bone formation in vivo. The current studies detail the development of a platform to advance SSC enrichment from human bone marrow, offering an invaluable resource for further SSC characterisation, with significant therapeutic impact therein.

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人类骨髓的单细胞 RNA 序列分析揭示了利用球形核酸分离骨骼干细胞的新目标。
大量数据表明,人类骨髓中含有骨骼干细胞(SSC),具有成骨、软骨和脂肪分化能力。然而,目前分离骨骼干细胞的方法因缺乏明确的标记物而受到限制,从而限制了对骨骼干细胞命运、免疫表型、功能和临床应用的了解。目前的研究应用单细胞 RNA 测序技术对来自 11 位捐献者的人类成人骨髓群进行了分析,并确定了用于 SSC 富集的新靶点。球形核酸用于检测 SSCs 中的这些 mRNA 靶点。这种方法能够快速分离出潜在的 SSC,其频率为
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来源期刊
Journal of Tissue Engineering
Journal of Tissue Engineering Engineering-Biomedical Engineering
CiteScore
11.60
自引率
4.90%
发文量
52
审稿时长
12 weeks
期刊介绍: The Journal of Tissue Engineering (JTE) is a peer-reviewed, open-access journal dedicated to scientific research in the field of tissue engineering and its clinical applications. Our journal encompasses a wide range of interests, from the fundamental aspects of stem cells and progenitor cells, including their expansion to viable numbers, to an in-depth understanding of their differentiation processes. Join us in exploring the latest advancements in tissue engineering and its clinical translation.
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