Evaluation of the immune checkpoint factors in idiopathic membranous nephropathy

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Roza Motavalli , Maryam Hosseini , Mohammad Sadegh Soltani-Zangbar , Abbas Karimi , Mohammadreza Sadeghi , Sanam Dolati , Mehdi Yousefi , Jalal Etemadi
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引用次数: 0

Abstract

Idiopathic membranous nephropathy (IMN), a single-organ autoimmune disease, is recognized by autoantibodies to podocyte proteins and identified as the most frequent cause of nephrotic syndrome in adults. T cells are important contributors in autoimmunity since they promote B–cell development, antibody production, direct inflammation, and organ tissue cytotoxicity. This study investigated the inhibitory immune checkpoint (ICP) receptors expressed on T lymphocytes and other immune cells. Thus, PBMCs from IMN patients were obtained before treatment, and the levels of ICPs such as programmed cell death protein 1 (PD-1), cytotoxic T-lymphocyte-associated protein 4 (CTLA4), lymphocyte activation gene-3 (LAG-3), and T cell immunoglobulin-3 (TIM-3) were examined at both gene and protein expression using real time PCR and Western blot tests respectively. The results illustrated that gene expression levels of ICPs reduced significantly in comparison to the control which were verified by related fold changes of protein expression sequentially. Our study revealed that CTLA-4, PD-1, TIM-3, and LAG-3 expression is impaired in IMN patients before treatment which could be a potential target for therapy.

免疫检查点因子在特发性膜性肾病中的评价
特发性膜性肾病(IMN)是一种单器官自身免疫性疾病,通过足细胞蛋白自身抗体识别,是成人肾病综合征最常见的病因。T细胞是自身免疫的重要贡献者,因为它们促进B细胞发育、抗体产生、直接炎症和器官组织细胞毒性。本研究研究了T淋巴细胞和其他免疫细胞上表达的抑制性免疫检查点(ICP)受体。因此,在治疗前获得来自IMN患者的PBMC,并分别使用实时PCR和Western印迹测试在基因和蛋白质表达方面检测诸如程序性细胞死亡蛋白1(PD-1)、细胞毒性T淋巴细胞相关蛋白4(CTLA4)、淋巴细胞活化基因-3(LAG-3)和T细胞免疫球蛋白-3(TIM-3)的ICP水平。结果表明,与对照相比,ICPs的基因表达水平显著降低,这通过蛋白质表达的相关倍数依次变化来验证。我们的研究表明,IMN患者在治疗前CTLA-4、PD-1、TIM-3和LAG-3的表达受损,这可能是治疗的潜在靶点。
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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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