Immunomodulatory effects of chronic trichinellosis on Toxoplasma gondii RH virulent strain in experimental rats.

IF 4.9 4区医学 Q1 PARASITOLOGY

Pathogens and Global Health Pub Date : 2023-06-01 Epub Date: 2023-03-15 DOI:10.1080/20477724.2023.2191233

Abeer E Saad, Dalia S Ashour, Eman Rashad

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引用次数: 0

Abstract

Mixed parasitic infections could affect the host immunological responses and re-design the pathogenesis of each other. The impact of Toxoplasma gondii (T. gondii) and Trichinella spiralis (T. spiralis) co-infection on the immune response remains unclear. The objective of the present study was to investigate the possible effect of chronic trichinellosis on the immune response of rats infected with T. gondii virulent RH strain. Animals were divided into four groups: group I: non-infected negative control; group II: infected with T. spiralis; group III: infected with T. gondii and group IV: infected with T. spiralis then infected with T. gondii 35 days post T. spiralis infection (co-infected group). The interaction between T. spiralis and T. gondii was evaluated by histopathological examination of liver and brain tissues, immunohistochemical expression of inducible nitric oxide synthase (iNOS), and β-catenin in the brain tissues, and CD4⁺ and CD8⁺ T cells percentages, and tumor necrosis factor (TNF)-alpha expression in the spleen tissues. Along with, splenic interleukin (IL)-4 and IL-10 mRNA expression levels were measured 15 days post-Toxoplasma infection. Our study revealed that prior infection with T. spiralis leads to attenuation of Th1 response against T. gondii, including iNOS, TNF-α, and CD8⁺ T-cell response with improvement of the histopathological changes in the tissues. In conclusion, in the co-infected rats, a balanced immune response has been developed with the end result, improvement of the histopathological changes in the liver and brain.

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慢性旋毛虫病对实验大鼠弓形虫 RH 毒株的免疫调节作用。

混合寄生虫感染会影响宿主的免疫反应，并重新设计彼此的发病机制。弓形虫（T. gondii）和旋毛虫（T. spiralis）混合感染对免疫反应的影响仍不清楚。本研究的目的是调查慢性旋毛虫病对感染了刚地弓形虫毒力 RH 株的大鼠的免疫反应可能产生的影响。动物被分为四组：I组：未感染阴性对照组；II组：感染螺旋体；III组：感染淋球菌；IV组：感染螺旋体后35天再感染淋球菌（共感染组）。通过肝脏和脑组织的组织病理学检查、脑组织中诱导型一氧化氮合酶（iNOS）和β-catenin的免疫组化表达、脾脏组织中CD4+和CD8+T细胞百分比以及肿瘤坏死因子（TNF）-α的表达来评估螺旋体和淋病双球菌之间的相互作用。此外，还测定了弓形虫感染后15天脾脏白细胞介素（IL）-4和IL-10 mRNA的表达水平。我们的研究表明，在感染弓形虫之前，螺旋体会导致针对弓形虫的 Th1 反应减弱，包括 iNOS、TNF-α 和 CD8+ T 细胞反应，组织病理学变化也会随之改善。总之，在合并感染的大鼠中，已经形成了一种平衡的免疫反应，其最终结果是改善了肝脏和大脑的组织病理学变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathogens and Global Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY

CiteScore

6.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Pathogens and Global Health is a journal of infectious disease and public health that focuses on the translation of molecular, immunological, genomics and epidemiological knowledge into control measures for global health threat. The journal publishes original innovative research papers, reviews articles and interviews policy makers and opinion leaders on health subjects of international relevance. It provides a forum for scientific, ethical and political discussion of new innovative solutions for controlling and eradicating infectious diseases, with particular emphasis on those diseases affecting the poorest regions of the world.