The acute effects of nonstructural-1 protein dengue virus type 2 on wet liver weight, zonulin expression and serum zonulin.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2023-04-01 DOI:10.18502/ijm.v15i2.12483

Safari Wahyu Jatmiko, Hartono Hartono, Tonang Dwi Ardyanto, Dono Indarto

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Abstract

Background and objectives: Intestinal leakage commonly occurs in severe dengue infection with zonulin as a biomarker. The aim of this study was to determine the effects of NS1 on liver weight, zonulin expression and serum zonulin levels.

Materials and methods: This laboratory experiment used 18 ddY mice, which were randomly divided into control (C), PBS (T1), and PBS + NS1 (T2) groups. Mice in the T1 and T2 groups were intravenously injected with 500 μl PBS only and 50 μg NS1 respectively. Mice blood samples were collected before and after three-day treatment for measurement of zonulin level. The fresh liver was weighted directly and were then used for immunostaining.

Results: The C group had lower wet liver weight compared to the T groups (p=0.001). Increased expression of liver zonulin was found in the T2 group, significant different from the C (p=0.014) and T1 groups (p=0.020). After treatment, serum zonulin levels in the T1 group was higher than that of the T1 group before treatment (p=0.035) but not in control (p=0.753) and T2 groups (p=0.869).

Conclusion: Administration of 50 μg NS 1 increases wet liver weight and zonulin expression in hepatocytes, but did not increase serum zonulin levels in ddY mice.

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登革病毒2型非结构1蛋白对湿肝重、带蛋白表达及血清带蛋白的急性影响。

背景和目的:肠漏常见于重症登革热感染，带胞蛋白为生物标志物。本研究的目的是确定NS1对肝脏重量、zonulin表达和血清zonulin水平的影响。材料与方法:本实验室实验选用18只ddY小鼠，随机分为对照组(C)、PBS组(T1)和PBS + NS1组(T2)。T1组和T2组小鼠分别静脉注射PBS 500 μl和NS1 50 μl。在给药前和给药后分别取小鼠血样，测定zonulin水平。新鲜肝脏直接称重，然后进行免疫染色。结果:C组湿肝重量低于T组(p=0.001)。T2组肝脏zonulin表达升高，与C组(p=0.014)和T1组(p=0.020)比较差异有统计学意义。治疗后，T1组患者血清zonulin水平高于治疗前T1组(p=0.035)，对照组(p=0.753)和T2组(p=0.869)差异不显著。结论:给药50 μg NS 1可增加小鼠肝湿重和肝细胞zonulin表达，但不增加血清zonulin水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.