Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania.

IF 1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

African Journal of Laboratory Medicine Pub Date : 2023-01-01 DOI:10.4102/ajlm.v12i1.2107

Jenipher G Mwakyabala, Conjester I Mtemisika, Stacy Mshana, Adam A Mwakyoma, Vitus Silago

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引用次数: 1

Abstract

Healthcare-associated infections (HCAIs) caused by extended spectrum β-lactamase-producing Gram-negative bacteria (ESBL-GNB) increase morbidity and mortality. This cross-sectional study characterised ESBL genes (bla _CTX-M, bla _TEM and bla _SHV) among 30 ceftriaxone-resistant GNB causing HCAIs between January 2022 and July 2022 by multiplex polymerase chain reaction assay at the zonal referral hospital in Mwanza, Tanzania. Twenty-five (83.3%) had at least one ESBL gene, of which 23/25 (92.0%) carried the bla _CTX-M gene. Seventy-two percent (18/25) of the GNB-ESBL isolates carried more than one ESBL gene, of which the majority (88.8%; n = 16/25) carried the bla _CTX-M and bla _TEM genes. Extended spectrum β-lactamase genes, particularly bla _CTX-M, are common among ceftriaxone-resistant GNB causing HCAIs.

What this study adds: This study revealed the distribution of genes (bla _CTX-M, bla _TEM and bla _SHV) coding for ESBL production among ceftriaxone resistant GNB causing HCAIs However, all ESBL producing GNB were susceptible towards ceftriaxone-sulbactam indicating that ceftriaxone-sulbactam may be empirically prescribed for treating patients with HCAIs.

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在坦桑尼亚姆万扎引起卫生保健相关感染的革兰氏阴性细菌中编码扩展谱β-内酰胺酶的基因特征

由广谱β-内酰胺酶产生的革兰氏阴性菌(ESBL-GNB)引起的医疗保健相关感染(HCAIs)增加了发病率和死亡率。这项横断面研究在坦桑尼亚姆万扎地区转诊医院通过多重聚合酶链反应测定，鉴定了2022年1月至2022年7月期间30例头孢曲松耐药GNB引起的HCAIs患者中的ESBL基因(bla CTX-M、bla TEM和bla SHV)。25例(83.3%)至少有1个ESBL基因，其中23/25(92.0%)携带bla CTX-M基因。72%(18/25)的GNB-ESBL分离株携带一个以上的ESBL基因，其中大多数(88.8%;n = 16/25)携带bla CTX-M和bla TEM基因。广谱β-内酰胺酶基因，特别是bla CTX-M，在头孢曲松耐药GNB引起的HCAIs中很常见。本研究补充:本研究揭示了在头孢曲松耐药GNB引起的HCAIs中编码产生ESBL的基因(bla CTX-M、bla TEM和bla SHV)的分布。然而，所有产生GNB的ESBL对头孢曲松-舒巴坦均敏感，提示头孢曲松-舒巴坦可作为治疗HCAIs患者的经经验处方。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

African Journal of Laboratory Medicine MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

1.70

自引率

9.10%

发文量

审稿时长

12 weeks

期刊介绍： The African Journal of Laboratory Medicine, the official journal of ASLM, focuses on the role of the laboratory and its professionals in the clinical and public healthcare sectors,and is specifically based on an African frame of reference. Emphasis is on all aspects that promote and contribute to the laboratory medicine practices of Africa. This includes, amongst others: laboratories, biomedical scientists and clinicians, medical community, public health officials and policy makers, laboratory systems and policies (translation of laboratory knowledge, practices and technologies in clinical care), interfaces of laboratory with medical science, laboratory-based epidemiology, laboratory investigations, evidence-based effectiveness in real world (actual) settings.