Denovo RNA-Seq analysis of ovary and testis reveals potential differentially expressed transcripts associated with gonadal unsynchronization development in Onychostoma macrolepis

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2023-03-01 DOI:10.1016/j.gep.2022.119303

Heran Cao , Long Li , Zhenpeng Li , Huihui Gao , Guofan Peng , Chao Zhu , Yining Chen , Fangxia Yang , Wuzi Dong

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引用次数: 0

Abstract

The Onychostoma macrolepis (O. macrolepis) is a rare and endangered wild species. Their endangered extinction might be due to their low fertility. To further illustrate the molecular mechanism of gonad development of the male and female O. macrolepis, the present study carried out de novo testicular and ovarian transcriptome sequencing. By comparing ovary and testis, 30,869 differentially expressed unigenes (9870 in female, 20999 in male) were identified. In addition, KEGG and GO analysis suggested that the Hedgehog signaling pathway have important roles in testis maintenance and spermatogenesis, whereas the Hippo signaling pathway and Wnt signaling pathway are likely to participate in ovary maintenance. RT-qPCR analysis results were consistent with transcriptome sequencing that all of gender differentiation-related genes (FOXL2, GDF9, WNT4, CYP19A1, SOX9 and GATA4), temperature-enriched genes (NOVA1, CTGF and NR4A1), clock-related genes (PER2, PER3, CRY1, CRY2, BMAL1 and CIPC) were significantly differential expression in testis compared with ovaries. Taken together, these results revealed a potential molecular mechanism that low fertility of the O. macrolepis might strong correlate with the gonadal dyssynchrony development of the male and female, which might provide theoretical basis and technical support for artificial reproduction and germplasm resource protection of the O. macrolepis.

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大鳞甲瘤卵巢和睾丸的Denovo RNA-Seq分析揭示了与性腺不同步发育相关的潜在差异表达转录物

大鳞甲鱼是一种珍稀濒危野生动物。它们濒临灭绝可能是由于它们的低生育能力。为了进一步阐明雄性和雌性大麻风杆菌性腺发育的分子机制，本研究进行了睾丸和卵巢转录组的从头测序。通过比较卵巢和睾丸，鉴定出30869个差异表达的unigenes（女性9870个，男性20999个）。此外，KEGG和GO分析表明，Hedgehog信号通路在睾丸维持和精子发生中具有重要作用，而Hippo信号通路和Wnt信号通路可能参与卵巢维持。RT-qPCR分析结果与转录组测序一致，所有性别分化相关基因（FOXL2、GDF9、WNT4、CYP19A1、SOX9和GATA4）、温度富集基因（NOVA1、CTGF和NR4A1）、时钟相关基因（PER2、PER3、CRY1、CRY2、BMAL1和CIPC）在睾丸中的表达与卵巢相比均有显著差异。总之，这些结果揭示了一个潜在的分子机制，即大鳞O.macrolepis的低生育能力可能与雄性和雌性性腺发育不同步密切相关，这可能为大鳞O.macrolepis的人工繁殖和种质资源保护提供理论依据和技术支持。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation