Fatty acid supplementation into warming solutions improves pregnancy outcomes after single vitrified-warmed cleavage stage embryo transfers.

IF 2.7 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Reproductive Medicine and Biology Pub Date : 2023-05-08 eCollection Date: 2023-01-01 DOI:10.1002/rmb2.12517

Ayumi Amagai, Kenji Ezoe, Tetsuya Miki, Kiyoe Shimazaki, Tadashi Okimura, Keiichi Kato

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Abstract

Purpose: This study aimed to examine the embryonic development of human 4-cell stage embryos after warming with fatty acids (FAs) and to assess the pregnancy outcomes after single vitrified-warmed cleavage stage embryo transfers (SVCTs).

Methods: Experimental study: A total of 217 discarded, vitrified human 4-cell stage embryos donated for research by consenting couples were used. The embryos were warmed using the fatty acid (FA)-supplemented solutions (FA group) or nonsupplemented solutions (control group). The developmental rate, morphokinetics, and outgrowth competence were analyzed. Clinical study: The treatment records of women undergoing SVCT in natural cycles between April and September 2022 were retrospectively analyzed (April-June 2022, control group; July-September 2022, FA group).

Results: Experimental study: The rate of morphologically good blastocysts was significantly higher in the FA group than in the control group (p = 0.0302). The morphokinetics during cleavage, morula, and blastocyst stages were comparable between the groups. The outgrowth was significantly increased in the FA group (p = 0.0438). Clinical study: The rates of implantation, clinical pregnancy, and ongoing pregnancy after SVCTs were significantly increased in the FA group (p = 0.0223-0.0281).

Conclusions: Fatty acid-supplemented warming solutions effectively improve embryo development to the blastocyst stage and pregnancy outcomes after SVCTs.

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在加温溶液中添加脂肪酸可改善单次玻璃化加温卵裂期胚胎移植后的妊娠结局。

目的：本研究旨在考察人类 4 细胞期胚胎经脂肪酸（FAs）升温后的胚胎发育情况，并评估单次玻璃化升温卵裂期胚胎移植（SVCTs）后的妊娠结局：实验研究：实验研究：共使用了 217 个经夫妇同意捐赠用于研究的废弃玻璃化人类 4 细胞期胚胎。使用脂肪酸（FA）补充溶液（FA 组）或非补充溶液（对照组）对胚胎进行加温。对胚胎的发育速度、形态动力学和生长能力进行分析。临床研究：回顾性分析2022年4月至9月期间自然周期接受SVCT的妇女的治疗记录（2022年4月至6月，对照组；2022年7月至9月，FA组）：实验研究：FA 组的囊胚形态良好率明显高于对照组（p = 0.0302）。各组在卵裂期、蜕膜期和囊胚期的形态动力学表现相当。FA组的胚胎长出明显增加（p = 0.0438）。临床研究：FA组在SVCT后的着床率、临床妊娠率和持续妊娠率明显增加（p = 0.0223-0.0281）：补充脂肪酸的温育液可有效改善胚胎发育至囊胚期，并改善 SVCT 后的妊娠结局。

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来源期刊

Reproductive Medicine and Biology Multiple-

CiteScore

5.70

自引率

5.90%

发文量

审稿时长

20 weeks

期刊介绍： Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.