Assessment of anti-arthritic activity of lipid matrix encased berberine in rheumatic animal model.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2023-06-01 DOI:10.1080/02652048.2023.2194414

Neelu Singh, Amit Kumar Pandey, Ravi Raj Pal, Alka, Poonam Parashar, Priya Singh, Nidhi Mishra, Dinesh Kumar, Ritu Raj, Sukhveer Singh, Shubhini A Saraf

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引用次数: 1

Abstract

The purpose of this study was to evaluate the drug delivery and therapeutic potential of berberine (Br) loaded nanoformulation in rheumatoid arthritis (RA)-induced animal model. The Br-loaded NLCs (nanostructured lipid carriers) were prepared employing melt-emulsification process, and optimised through Box-Behnken design. The prepared NLCs were assessed for in-vitro and in-vivo evaluations. The optimised NLCs exhibited a mean diameter of 180.2 ± 0.31 nm with 88.32 ± 2.43% entrapment efficiency. An enhanced anti-arthritic activity with reduced arthritic scores to 0.66 ± 0.51, reduction in ankle diameter to 5.80 ± 0.27 mm, decline in paw withdrawal timing, and improvements in walking behaviour were observed in the Br-NLCs treated group. The radiographic images revealed a reduction in bone and cartilage deformation. The Br-NLCs showed promising results in the management of RA disease, can be developed as an efficient delivery system at commercial levels, and may be explored for clinical application after suitable experiments in the future.

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类风湿动物模型中脂质基质包膜小檗碱抗关节炎活性的评价。

本研究的目的是评估小檗碱(Br)负载纳米制剂在类风湿关节炎(RA)诱导动物模型中的药物传递和治疗潜力。采用熔融乳化法制备硼负载的纳米脂质载体，并通过Box-Behnken设计对其进行优化。对制备的NLCs进行体外和体内评价。优化后的nlc平均直径为180.2±0.31 nm，包封效率为88.32±2.43%。Br-NLCs治疗组抗关节炎活性增强，关节炎评分降至0.66±0.51，踝关节直径降至5.80±0.27 mm，足部退断时间缩短，行走行为改善。x线图像显示骨和软骨变形减少。Br-NLCs在RA疾病的治疗中显示出良好的效果，可作为一种高效的商业给药系统开发，并可在未来通过适当的实验探索临床应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.