Primary infection of BALB/c mice with a dengue virus type 4 strain leads to kidney injury.

IF 2.5 4区 医学 Q2 PARASITOLOGY
Arthur da Costa Rasinhas, Fernanda Cunha Jácome, Gabriela Cardoso Caldas, Ana Luisa Teixeira de Almeida, Daniel Dias Coutinho de Souza, João Paulo Rodrigues Dos Santos, Helver Gonçalves Dias, Eduarda Lima Araujo, Ronaldo Mohana-Borges, Ortrud Monika Barth, Flavia Barreto Dos Santos, Debora Ferreira Barreto-Vieira
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Abstract

Background: Dengue is a disease caused by dengue virus (DENV-1 through -4). Among the four serotypes, DENV-4 remains the least studied. Acute kidney injury is a potential complication of dengue generally associated with severe dengue infection.

Objectives: The goal of this study was to investigate the alterations caused by experimental dengue infection in the kidney of adult BALB/c mice.

Methods: In this study, BALB/c mice were infected through the intravenous route with a DENV-4 strain, isolated from a human patient. The kidneys of the mice were procured and subject to histopathological and ultrastructural analysis.

Findings: The presence of the viral antigen was confirmed through immunohistochemistry. Analysis of tissue sections revealed the presence of inflammatory cell infiltrate throughout the parenchyma. Glomerular enlargement was a common find. Necrosis of tubular cells and haemorrhage were also observed. Analysis of the kidney on a transmission electron microscope allowed a closer look into the necrotic tubular cells, which presented nuclei with condensed chromatin, and loss of cytoplasm.

Main conclusions: Even though the kidney is probably not a primary target of dengue infection in mice, the inoculation of the virus in the blood appears to damage the renal tissue through local inflammation.

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BALB/c小鼠原发性感染登革病毒4型毒株可导致肾脏损伤。
背景:登革热是由登革病毒(DENV-1至-4)引起的疾病。在四种血清型中,DENV-4仍然是研究最少的。急性肾损伤是登革热的潜在并发症,通常与严重登革热感染有关。目的:本研究的目的是研究实验性登革热感染对成年BALB/c小鼠肾脏的影响。方法:在本研究中,BALB/c小鼠通过静脉途径感染从人类患者分离的DENV-4菌株。取小鼠肾脏,进行组织病理学和超微结构分析。结果:免疫组化证实存在病毒抗原。组织切片分析显示炎性细胞浸润于整个实质。肾小球增大是常见的。小管细胞坏死,出血。在透射电子显微镜下对肾脏进行分析,可以更仔细地观察坏死的小管细胞,其细胞核染色质浓缩,细胞质丢失。主要结论:尽管肾脏可能不是小鼠登革热感染的主要目标,但在血液中接种病毒似乎通过局部炎症损害肾脏组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.00
自引率
3.60%
发文量
91
审稿时长
3-8 weeks
期刊介绍: Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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