Associations Between Blood Levels of NLRP3 Inflammasome Components and Obsessive Compulsive Disorder.

IF 1 4区医学 Q4 CLINICAL NEUROLOGY

Noropsikiyatri Arsivi-Archives of Neuropsychiatry Pub Date : 2023-02-26 eCollection Date: 2023-01-01 DOI:10.29399/npa.28127

Melike Tetik, Neşe Direk, Betül Önder Uzgan, Cansu Aykaç, Burcu Ekinci, Tutku Yaraş, Aykut Kuruoğlu, Fatih Özel, Çağatay Ermiş, Tunç Alkın, Yavuz Oktay

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引用次数: 0

Abstract

Introduction: Even though the effect of inflammation on pathogenesis of obsessive compulsive disorder (OCD) is known, information regarding the underlying mechanisms are yet to be revealed. The NLRP3 inflammasome complex is an important component of the innate immune system that initiates and mediates inflammatory response to a variety of stimuli. This study aims to inquire into a possible association between NLRP3 inflammasome complex and OCD.

Methods: This case-control study included 103 participants (51 cases with OCD and 52 healthy controls). All participants were evaluated with the Yale Brown Obsessive Compulsive Scale, Hamilton Depression Scale, and Hewitt Multidimensional Perfectionism Scale. RNA and proteins were extracted from peripheral blood mononuclear cells. Expression of NLRP3 inflammasome components were determined using quantitative real-time polymerase chain reaction (PCR) and Western blotting. Levels of Serum IL-1beta and IL-18 cytokine were determined by ELISA.

Results: NEK7 and CASP1 mRNA levels were significantly higher in OCD patients, compared to controls. Pro-caspase-1 protein levels were elevated, as well. Regression analysis showed that NEK7 mRNA and pro-caspase-1 protein levels can differentiate OCD and healthy control groups.

Conclusion: Our results provide insight into the molecular alterations that could explain the inflammation-OCD association.

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血液中 NLRP3 炎症组成分的水平与强迫症之间的关系

导言：尽管炎症对强迫症（OCD）发病机制的影响已众所周知，但有关其潜在机制的信息仍有待揭示。NLRP3 炎症小体复合物是先天性免疫系统的一个重要组成部分，可启动和介导对各种刺激的炎症反应。本研究旨在探讨NLRP3炎性体复合体与强迫症之间可能存在的关联：这项病例对照研究包括 103 名参与者（51 名强迫症患者和 52 名健康对照者）。所有参与者均接受了耶鲁布朗强迫症量表、汉密尔顿抑郁量表和休伊特多维完美主义量表的评估。从外周血单核细胞中提取了 RNA 和蛋白质。采用定量实时聚合酶链反应（PCR）和 Western 印迹法测定 NLRP3 炎性体成分的表达。用 ELISA 法测定血清 IL-1beta 和 IL-18 细胞因子的水平：结果：与对照组相比，强迫症患者的NEK7和CASP1 mRNA水平明显升高。Pro-caspase-1 蛋白水平也有所升高。回归分析表明，NEK7 mRNA和原caspase-1蛋白水平可以区分强迫症组和健康对照组：我们的研究结果为解释炎症与强迫症的关联提供了分子改变的线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Noropsikiyatri Arsivi-Archives of Neuropsychiatry CLINICAL NEUROLOGY-

CiteScore

1.70

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Archives of Neuropsychiatry (Arch Neuropsychiatry) is the official journal of the Turkish Neuropsychiatric Society. It is published quarterly, and four editions annually constitute a volume. Archives of Neuropsychiatry is a peer reviewed scientific journal that publishes articles on psychiatry, neurology, and behavioural sciences. Both clinical and basic science contributions are welcomed. Submissions that address topics in the interface of neurology and psychiatry are encouraged. The content covers original research articles, reviews, letters to the editor, and case reports.