High diversity of yeast species and strains responsible for vulvovaginal candidiasis in South-East Gabon

IF 2.2 4区医学 Q3 MYCOLOGY

Journal de mycologie medicale Pub Date : 2023-05-01 DOI:10.1016/j.mycmed.2022.101354

M. Bignoumba , R. Onanga , B.S. Kumulungui , R.F. Kassa Kassa , Y. Mouanga Ndzime , K. Mbombe Moghoa , D. Stubbe , P. Becker

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引用次数: 0

Abstract

Objectives

Candida albicans generally remains the principal pathogenic yeast responsible for vulvovaginal candidiasis (VVC), although with variable prevalence. In this study, we evaluated the evolution of the prevalence of the non-Candida albicans Candida (NCAC) species and investigated the genotypic diversity and the population genetic structure of the circulating C. albicans strains associated with VVC in the vicinity of Franceville (Gabon).

Methods

A total of 110 independent isolates were identified using both MALDI-TOF MS and conventional techniques. The population genetic structure of the C. albicans strains was determined by multiple locus variable-number tandem repeat analysis using 4 microsatellite markers.

Results

The mean and median age of the patients was 31 years. Seven patients had a mixed infection. C. albicans accounted for 62 % (n=68) of the total isolates. NCAC were dominated by C. glabrata, followed by P. kudriavzevii, C. parapsilosis, C. tropicalis, M. guilliermondii, and C. nivariensis. The cluster analysis revealed a high diversity, with a total of 50 different genotypes. The most represented genotype was shared by only four strains, while the vast majority (39 strains) had a unique MLVA pattern. Geographic clusters were not detected.

Conclusion

The study provides information on species distribution and possible changing epidemiology while reporting for the first time C. nivariensis in VVC in Africa. This study is also the first to investigate the genotypic diversity of the circulating C. albicans strains associated with VVC in Central Africa. Such analyses would help understand the molecular epidemiology of C. albicans.

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加蓬东南部引起外阴阴道念珠菌病的酵母菌种类和菌株的高度多样性

目的白色念珠菌通常仍然是导致外阴阴道念珠菌病（VVC）的主要致病酵母，尽管其患病率各不相同。在本研究中，我们评估了非白色念珠菌Candida（NCAC）物种的流行演变，并调查了在加蓬Franceville附近与VVC相关的循环白色念珠菌菌株的基因型多样性和群体遗传结构。利用4个微卫星标记，通过多基因座可变数串联重复分析确定了白色念珠菌菌株的群体遗传结构。结果患者的平均和中位年龄为31岁。7名患者有混合感染。白色念珠菌占分离株总数的62%（n=68）。NCAC主要由光滑乳杆菌（C.glabrata）为主，其次是库氏乳杆菌（P.kudriavzevii）、副psilosis乳杆菌、热带乳杆菌（C.tropicalis）、吉列蒙迪乳杆菌（M.guilliermondii）和尼瓦里乳杆菌（C.nivariensis）。聚类分析显示出高度多样性，共有50种不同的基因型。最具代表性的基因型仅由四个菌株共享，而绝大多数（39个菌株）具有独特的MLVA模式。未检测到地理群集。结论该研究首次报道了非洲VVC中的雪腐镰刀菌的物种分布和可能的流行病学变化。这项研究也是首次调查中非与VVC相关的循环白色念珠菌菌株的基因型多样性。这样的分析将有助于了解白色念珠菌的分子流行病学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal de mycologie medicale 医学-真菌学

CiteScore

5.10

自引率

2.80%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal de Mycologie Medicale / Journal of Medical Mycology (JMM) publishes in English works dealing with human and animal mycology. The subjects treated are focused in particular on clinical, diagnostic, epidemiological, immunological, medical, pathological, preventive or therapeutic aspects of mycoses. Also covered are basic aspects linked primarily with morphology (electronic and photonic microscopy), physiology, biochemistry, cellular and molecular biology, immunochemistry, genetics, taxonomy or phylogeny of pathogenic or opportunistic fungi and actinomycetes in humans or animals. Studies of natural products showing inhibitory activity against pathogenic fungi cannot be considered without chemical characterization and identification of the compounds responsible for the inhibitory activity. JMM publishes (guest) editorials, original articles, reviews (and minireviews), case reports, technical notes, letters to the editor and information. Only clinical cases with real originality (new species, new clinical present action, new geographical localization, etc.), and fully documented (identification methods, results, etc.), will be considered. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey platforms.