The possible effect of lycopene in ameliorating experimentally induced ulcerative colitis in adult male albino rats (A histological, immunohistochemical, and ultrastructural study).

IF 1.1 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2023-05-04 Epub Date: 2023-03-03 DOI:10.1080/01913123.2023.2185718

Shaimaa Mostafa Kashef, Rania Ibrahim Yassien, Dalia El-Sayed El-Ghazouly

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引用次数: 0

Abstract

Ulcerative colitis (UC) is considered a long-term inflammatory disorder worldwide. Its pathogenesis is associated with reduced antioxidant capacity. Lycopene (LYC) is a powerful antioxidant with strong free radical scavenging property. The present work has done to assess changes of colonic mucosa in induced UC and the possible ameliorative effects of LYC. Forty-five adult male albino rats were randomly divided into four groups: group I (control), group II was given 5 mg/kg/day (LYC) by oral gavage for 3 weeks. Group III (UC) was received single intra-rectal injection of acetic acid. Group IV (LYC+UC) received LYC in same dose and duration as before and acetic acid on 14th day of the experiment. UC group showed loss of surface epithelium with destructed crypts. Congested blood vessels with heavy cellular infiltration were observed. Significant decrease in goblet cell numbers and the mean area percentage of ZO-1 immunoexpression were noticed. Significant increase in the mean area percentage of collagen and the mean area percentage of COX-2 were also noticed. Ultrastructural changes were matched with light microscopic results that showed abnormal destructive columnar and goblet cells. Histological, immunohistochemical, and ultrastructural findings in group IV supported the ameliorative role of LYC against destructive changes induced by UC.

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番茄红素对改善成年雄性白化大鼠实验性溃疡性结肠炎的可能影响（组织学、免疫组织化学和超微结构研究）。

溃疡性结肠炎（UC）被认为是一种全球性的长期炎症性疾病。其发病机制与抗氧化能力下降有关。番茄红素（LYC）是一种强大的抗氧化剂，具有很强的清除自由基的能力。本研究旨在评估 UC 诱导的结肠粘膜变化以及 LYC 可能的改善作用。将 45 只成年雄性白化大鼠随机分为四组：第一组（对照组），第二组口服 5 毫克/千克/天（LYC），连续 3 周。第三组（UC）接受单次乙酸直肠内注射。第四组（LYC+UC）在实验的第 14 天接受与之前相同剂量和时间的 LYC 和醋酸。UC 组显示表面上皮脱落，隐窝破坏。观察到有大量细胞浸润的充血血管。注意到鹅口疮细胞数量和 ZO-1 免疫表达的平均面积百分比显著下降。还发现胶原蛋白的平均面积百分比和 COX-2 的平均面积百分比显著增加。超微结构的变化与光学显微镜的结果相吻合，后者显示出异常的破坏性柱状细胞和小管细胞。第 IV 组的组织学、免疫组化和超微结构研究结果表明，LYC 对 UC 引起的破坏性变化有改善作用。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.