Automation in flow cytometry: Guidelines and review of systems

IF 2.3 3区医学 Q3 MEDICAL LABORATORY TECHNOLOGY

Cytometry Part B: Clinical Cytometry Pub Date : 2023-05-13 DOI:10.1002/cyto.b.22125

Ahmad Al-Attar, Kirthi R. Kumar, Dorian Untersee, Marci O'Driscoll, Miguel Francoise S. Ventura, Leo Lin

引用次数: 0

Abstract

Automation in flow cytometry has recently advanced from the partial laboratory automation and robotics islets, to more fully integrated systems. This article reviews three manufacturers' newest sample preparation systems: the Beckman CellMek, the Sysmex PS-10, and the BD FACSDuet. These three instruments are capable of performing many of the manual steps in flow cytometry sample processing (pipetting, staining, lysing, washing, fixing). General description, capabilities, advantages, and disadvantages of each system are compared. Overall, these systems have the potential to become mainstay items in today's busy clinical flow cytometry laboratories, and save a significant amount of hands-on time for laboratory staff.

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流式细胞仪自动化：系统指南与回顾。

最近，流式细胞仪的自动化已从部分实验室自动化和机器人系统发展到更全面的集成系统。本文评述了三家制造商的最新样品制备系统：Beckman CellMek、Sysmex PS-10 和 BD FACSDuet。这三种仪器能够执行流式细胞仪样本处理过程中的许多手动步骤（移液、染色、裂解、洗涤、固定）。对每种系统的一般描述、功能、优缺点进行了比较。总之，这些系统有可能成为当今繁忙的临床流式细胞仪实验室的主要设备，为实验室工作人员节省大量的动手时间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytometry Part B: Clinical Cytometry 医学-病理学

CiteScore

6.80

自引率

32.40%

发文量

审稿时长

>12 weeks

期刊介绍： Cytometry Part B: Clinical Cytometry features original research reports, in-depth reviews and special issues that directly relate to and palpably impact clinical flow, mass and image-based cytometry. These may include clinical and translational investigations important in the diagnostic, prognostic and therapeutic management of patients. Thus, we welcome research papers from various disciplines related [but not limited to] hematopathologists, hematologists, immunologists and cell biologists with clinically relevant and innovative studies investigating individual-cell analytics and/or separations. In addition to the types of papers indicated above, we also welcome Letters to the Editor, describing case reports or important medical or technical topics relevant to our readership without the length and depth of a full original report.