Construction of homozygous diploid potato through maternal haploid induction

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jinzhe Zhang, Jian Yin, Jiayi Luo, Die Tang, Xijian Zhu, Jie Wang, Zhihong Liu, Pei Wang, Yu Zhong, Chenxu Liu, Canhui Li, Shaojiang Chen, Sanwen Huang
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引用次数: 6

Abstract

Reinventing the tetraploid potato into a seed-propagated, diploid, hybrid potato would significantly accelerate potato breeding. In this regard, the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes, but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines. To resolve this impediment, we developed a doubled haploid (DH) technology, based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (StDMP) gene. Here, we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines. An exercisable protocol, involving hybridization, fluorescent marker screening, molecular and flow cytometric identification, and doubling with colchicine generates nearly 100% homozygous diploid potato lines. This dmp-triggered haploid induction (HI) system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.

Abstract Image

通过母体单倍体诱导构建纯合二倍体马铃薯
将四倍体马铃薯改造成种子繁殖的二倍体杂交马铃薯将显著加快马铃薯育种。在这方面,发展高度纯合的自交系是培育杂交马铃薯的先决条件,但自交不亲和和近交抑制对发展纯自交系提出了挑战。为了解决这一障碍,我们开发了一种基于马铃薯未知功能域679膜蛋白(StDMP)基因突变的双单倍体(DH)技术。在这里,我们表明StDMP的缺乏允许产生用于产生二倍体马铃薯系的母体单倍体。一种可行的方案,包括杂交、荧光标记筛选、分子和流式细胞仪鉴定,以及用秋水仙碱加倍,可以产生近100%纯合的二倍体马铃薯系。这种dmp触发的单倍体诱导(HI)系统大大缩短了育种过程,为培育高纯度的二倍体马铃薯自交系提供了一种强有力的方法。
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来源期刊
CiteScore
7.70
自引率
2.80%
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