Construction of homozygous diploid potato through maternal haploid induction

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jinzhe Zhang, Jian Yin, Jiayi Luo, Die Tang, Xijian Zhu, Jie Wang, Zhihong Liu, Pei Wang, Yu Zhong, Chenxu Liu, Canhui Li, Shaojiang Chen, Sanwen Huang
{"title":"Construction of homozygous diploid potato through maternal haploid induction","authors":"Jinzhe Zhang,&nbsp;Jian Yin,&nbsp;Jiayi Luo,&nbsp;Die Tang,&nbsp;Xijian Zhu,&nbsp;Jie Wang,&nbsp;Zhihong Liu,&nbsp;Pei Wang,&nbsp;Yu Zhong,&nbsp;Chenxu Liu,&nbsp;Canhui Li,&nbsp;Shaojiang Chen,&nbsp;Sanwen Huang","doi":"10.1007/s42994-022-00080-7","DOIUrl":null,"url":null,"abstract":"<div><p>Reinventing the tetraploid potato into a seed-propagated, diploid, hybrid potato would significantly accelerate potato breeding. In this regard, the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes, but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines. To resolve this impediment, we developed a doubled haploid (DH) technology, based on mutagenesis of the potato <i>DOMAIN OF UNKNOWN FUNCTION 679 membrane protein</i> (<i>StDMP</i>) gene. Here, we show that a deficiency in <i>StDMP</i> allows the generation of maternal haploids for generating diploid potato lines. An exercisable protocol, involving hybridization, fluorescent marker screening, molecular and flow cytometric identification, and doubling with colchicine generates nearly 100% homozygous diploid potato lines. This <i>dmp</i>-triggered haploid induction (HI) system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"3 3","pages":"163 - 168"},"PeriodicalIF":4.6000,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-022-00080-7.pdf","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"aBIOTECH","FirstCategoryId":"1091","ListUrlMain":"https://link.springer.com/article/10.1007/s42994-022-00080-7","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 6

Abstract

Reinventing the tetraploid potato into a seed-propagated, diploid, hybrid potato would significantly accelerate potato breeding. In this regard, the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes, but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines. To resolve this impediment, we developed a doubled haploid (DH) technology, based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (StDMP) gene. Here, we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines. An exercisable protocol, involving hybridization, fluorescent marker screening, molecular and flow cytometric identification, and doubling with colchicine generates nearly 100% homozygous diploid potato lines. This dmp-triggered haploid induction (HI) system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.

Abstract Image

通过母体单倍体诱导构建纯合二倍体马铃薯
将四倍体马铃薯改造成种子繁殖的二倍体杂交马铃薯将显著加快马铃薯育种。在这方面,发展高度纯合的自交系是培育杂交马铃薯的先决条件,但自交不亲和和近交抑制对发展纯自交系提出了挑战。为了解决这一障碍,我们开发了一种基于马铃薯未知功能域679膜蛋白(StDMP)基因突变的双单倍体(DH)技术。在这里,我们表明StDMP的缺乏允许产生用于产生二倍体马铃薯系的母体单倍体。一种可行的方案,包括杂交、荧光标记筛选、分子和流式细胞仪鉴定,以及用秋水仙碱加倍,可以产生近100%纯合的二倍体马铃薯系。这种dmp触发的单倍体诱导(HI)系统大大缩短了育种过程,为培育高纯度的二倍体马铃薯自交系提供了一种强有力的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.70
自引率
2.80%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信