Characterization of the Trr/Trx system in the fungal pathogen Candida glabrata

IF 2.4 3区生物学 Q3 GENETICS & HEREDITY

Fungal Genetics and Biology Pub Date : 2023-05-01 DOI:10.1016/j.fgb.2023.103799

Guadalupe Gutiérrez-Escobedo , Norma Vázquez-Franco , Ana López-Marmolejo , Gabriel Luna-Arvizu , Israel Cañas-Villamar , Irene Castaño , Alejandro De Las Peñas

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Abstract

C. glabrata, an opportunistic fungal pathogen, can adapt and resist to different stress conditions. It is highly resistant to oxidant stress compared to other Candida spp and to the phylogenetically related but non-pathogen Saccharomyces cerevisiae. In this work, we describe the Trx/Trr system of C. glabrata composed of Trr1 and Trr2 (thioredoxin reductases) and Trx2 (thioredoxin) that are localized in the cytoplasm and Trx3 present in the mitochondrion. The transcriptional induction of TRR2 and TRX2 by oxidants depends on Yap1 and Skn7 and TRR1 and TRX3 have a low expression level. Both TRR2 and TRX2 play an important role in the oxidative stress response. The absence of TRX2 causes auxotrophy of methionine and cysteine. Trr1 and Trr2 are necessary for survival at high temperatures and for the chronological life span of C. glabrata. Furthermore, the Trx/Trr system is needed for survival in the presence of neutrophils. The role of TRR1 and TRX3 is not clear, but in the presence of neutrophils, they have non-overlapping functions with their TRR2 and TRX2 paralogues.

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光秃念珠菌Trr/Trx系统的研究

青霉(C. glabrata)是一种条件性真菌病原体，能够适应和抵抗不同的胁迫条件。与其他念珠菌相比，它对氧化胁迫具有很强的抗性，对系统发育相关但非病原体酿酒酵母具有很强的抗性。在这项工作中，我们描述了由Trr1和Trr2(硫氧还蛋白还原酶)和Trx2(硫氧还蛋白还原酶)组成的C. glabrata的Trx/Trr系统，Trx3存在于线粒体中。氧化剂对TRR2和TRX2的转录诱导依赖于Yap1和Skn7, TRR1和TRX3的表达水平较低。TRR2和TRX2在氧化应激反应中都起重要作用。TRX2的缺失导致蛋氨酸和半胱氨酸的萎缩。Trr1和Trr2在高温下的生存和对光棘草的年代寿命都是必需的。此外，在中性粒细胞存在的情况下，Trx/Trr系统是存活所必需的。TRR1和TRX3的作用尚不清楚，但在中性粒细胞存在时，它们与TRR2和TRX2同源物具有不重叠的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fungal Genetics and Biology 生物-遗传学

CiteScore

6.20

自引率

3.30%

发文量

审稿时长

85 days

期刊介绍： Fungal Genetics and Biology, formerly known as Experimental Mycology, publishes experimental investigations of fungi and their traditional allies that relate structure and function to growth, reproduction, morphogenesis, and differentiation. This journal especially welcomes studies of gene organization and expression and of developmental processes at the cellular, subcellular, and molecular levels. The journal also includes suitable experimental inquiries into fungal cytology, biochemistry, physiology, genetics, and phylogeny. Fungal Genetics and Biology publishes basic research conducted by mycologists, cell biologists, biochemists, geneticists, and molecular biologists. Research Areas include: • Biochemistry • Cytology • Developmental biology • Evolutionary biology • Genetics • Molecular biology • Phylogeny • Physiology.