Untargeted metabolomics reveals altered branch chain amino acids, glucose and fat metabolism contributing to coronary artery disease among Indian diabetic patients†

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2023-01-31 DOI:10.1039/D2MO00320A

Ramu Adela, Siva Swapna Kasarla, Najmuddin Saquib, Sonu Kumar Gupta, Sneh Bajpai, Yashwant Kumar and Sanjay K Banerjee

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引用次数: 0

Abstract

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterised by increased blood glucose levels. Patients with T2DM have a high risk of developing atherosclerotic coronary artery disease (CAD). CAD with T2DM has a complex etiology and the understanding of the pathophysiology of coronary artery disease (CAD) in the presence of diabetes is poor. Here, we have used LC-MS/MS-based untargeted metabolomics to unveil the alterations of metabolites in the serum of South-Indian patients diagnosed with T2DM, CAD and T2DM along with CAD (T2DM-CAD) compared with the healthy subjects (CT). Using untargeted metabolomics and network-based approaches, a set of metabolites highly co-expressed with T2DM-CAD pathogenesis were identified. Our results revealed that these metabolites belong to essential pathways such as amino acid metabolism, fatty acid metabolism and carbohydrate metabolism. The candidate metabolites identified by metabolomics study are branch chain amino acids, L-arginine, linoleic acid, L-serine, L-cysteine, fructose-6-phosphate, glycerol, creatine and 3-phosphoglyceric acid, and explain the pathogenesis of T2DM-assisted CAD. The identified metabolites could be used as potential prognostic markers to predict CAD in patients diagnosed with T2DM.

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非靶向代谢组学揭示了支链氨基酸、葡萄糖和脂肪代谢的改变与印度糖尿病患者冠状动脉疾病有关†

2型糖尿病(T2DM)是一种以血糖水平升高为特征的慢性代谢紊乱。T2DM患者发生动脉粥样硬化性冠状动脉疾病(CAD)的风险很高。冠心病合并2型糖尿病的病因复杂，目前对冠心病合并糖尿病的病理生理了解较少。在这里，我们使用LC-MS/MS-based非靶向代谢组学来揭示诊断为T2DM, CAD和T2DM合并CAD (T2DM-CAD)的南印度患者与健康受试者(CT)相比血清中代谢物的变化。利用非靶向代谢组学和基于网络的方法，确定了一组与T2DM-CAD发病机制高度共表达的代谢物。结果表明，这些代谢产物属于氨基酸代谢、脂肪酸代谢和碳水化合物代谢等必需途径。代谢组学研究确定的候选代谢物有支链氨基酸、l -精氨酸、亚油酸、l -丝氨酸、l -半胱氨酸、6-磷酸果糖、甘油、肌酸和3-磷酸甘油酸，这些代谢物解释了t2dm辅助CAD的发病机制。鉴定出的代谢物可作为预测T2DM患者CAD的潜在预后标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.