Loading Ovalbumin into Mesenchymal Stem Cell-Derived Exosomes as a Nanoscale Carrier with Immunomodulatory Potential for Allergen-Specific Immunotherapy.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2023-01-01 DOI:10.52547/rbmb.11.4.626

Sajad Dehnavi, Ali Khodadadi, Ali Asadirad, Ata Ghadiri

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引用次数: 1

Abstract

Background: Exosomes are nanoscale vesicles widely used as drug delivery systems. Mesenchymal stem cell (MSC)-derived exosomes have shown immunomodulatory potential. This study optimized loading OVA into the mice adipose tissue-derived MSC-isolated exosomes to prepare the OVA-MSC-exosome complex for allergen-specific immunotherapy.

Methods: MSCs were harvested from mice adipose tissue and characterized by flow cytometry and evaluating differentiation potential. The exosomes were isolated and characterized via Dynamic Light Scattering, Scanning Electron Microscopy, and flow cytometry. Different concentrations of ovalbumin were incubated with MSC-exosome in various durations to optimize a more suitable protocol. BCA and HPLC analysis were used to quantify, and DLS was applied to qualify the prepared formulation of the OVA-exosome complex.

Results: The harvested MSCs and isolated exosomes were characterized. Analysis of the OVA-exosome complex revealed that OVA in primary 500 μg/ml concentration and incubation for 6 h results in higher efficacy.

Conclusions: Loading OVA into MSC-derived exosomes was successfully optimized and could be administrated for allergen-specific immunotherapy in the animal model.

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将卵清蛋白装载到间充质干细胞衍生的外泌体中，作为具有过敏原特异性免疫治疗免疫调节潜力的纳米载体。

背景:外泌体是一种纳米级囊泡，广泛用于药物传递系统。间充质干细胞(MSC)衍生的外泌体显示出免疫调节潜力。本研究优化了将OVA加载到小鼠脂肪组织来源的msc分离外泌体中，以制备OVA- msc -外泌体复合物，用于过敏原特异性免疫治疗。方法:从小鼠脂肪组织中获取间充质干细胞，采用流式细胞术对其进行鉴定并评估其分化潜力。外泌体分离并通过动态光散射、扫描电镜和流式细胞术进行表征。不同浓度的卵清蛋白与msc -外泌体在不同时间内孵育，以优化更合适的方案。使用BCA和HPLC分析进行定量，并使用DLS对ova -外泌体复合物的制备配方进行鉴定。结果:收获的间充质干细胞和分离的外泌体被鉴定。OVA-外泌体复合物分析表明，OVA初始浓度为500 μg/ml，孵育6 h，效果较好。结论:成功优化了将OVA加载到msc衍生的外泌体中，可用于动物模型的过敏原特异性免疫治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.