Detection of different colistin resistance mechanisms among multidrug resistant Klebsiella pneumoniae isolates in Bulgaria.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-09-16 DOI:10.1556/030.2022.01746

Rumyana Markovska, Yuliya Marteva-Proevska, Tzvetan Velinov, Ivan Pavlov, Radka Kaneva, Lyudmila Boyanova

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引用次数: 1

Abstract

The more frequent usage of colistin resulted in an increase of colistin resistance due to lipopolysaccharide modifications. The aim of this study was to reveal the prevalence and mechanisms of colistin resistance among multidrug-resistant Klebsiella pneumoniae isolates collected in Bulgaria. One hundred multidrug resistant K. pneumoniae isolates were collected in a period between 2017 and 2018. Among them, 29 colistin resistant and 8 heteroresistant isolates were observed and further investigated. Clonal relatedness was detected by RAPD and MLST. Сarbapenemases, two component system phoQ/phoP, pmrA/B, and mgrB were investigated by PCR amplification and Sanger sequencing. Among 37 colistin nonsusceptible isolates, we detected 25 NDM-1 producers. The isolates belonged mainly to ST11 (80%), and also to ST147, ST35, ST340, ST219 (1-2 members per clone). Nine colistin resistant isolates showed changes in mgrB. IS903B-like elements truncated mgrB in five isolates. In two isolates, premature stopcodon (Q30stopcodon) was observed and another two isolates did not amplify mgrB, possibly due to bigger deletion or insertion. No isolates showed phoQ/phoP and pmrA/B mutations except for pmrB (four isolates had R256G). All isolates with IS903B insertions belonged to ST11 clone. The mgrB alterations play major role in colistin resistance in K. pneumoniae isolates studied in the current work. We report truncation of mgrB by IS903 like element in colistin resistant NDM-1 producing K. pneumoniae ST11 clone in Bulgaria.

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保加利亚多重耐药肺炎克雷伯菌分离株不同粘菌素耐药机制的检测。

粘菌素的使用频率越高，由于脂多糖修饰导致粘菌素耐药性增加。本研究的目的是揭示在保加利亚收集的多重耐药肺炎克雷伯菌分离株中粘菌素耐药性的患病率和机制。在2017年至2018年期间收集了100株多重耐药肺炎克雷伯菌分离株。其中29株对粘菌素耐药，8株对异源耐药。克隆亲缘关系通过RAPD和MLST检测。Сarbapenemases，双组分体系phoQ/phoP, pmrA/B和mgrB通过PCR扩增和Sanger测序进行研究。在37株粘菌素不敏感的分离株中，检测到25株NDM-1产生菌。分离株主要为ST11(80%)，也有ST147、ST35、ST340、ST219(1 ~ 2个)。9株耐粘菌素菌株的mgrB出现变化。在5个分离株中，is903b样元件截断了mgrB。在两个分离株中观察到过早终止密码子(q30终止密码子)，另外两个分离株没有扩增mgrB，可能是由于更大的缺失或插入。除pmrB外，没有分离株出现phoQ/phoP和pmrA/B突变(4株有R256G)。所有插入IS903B的分离株均属于ST11克隆。mgrB的改变在目前研究的肺炎克雷伯菌分离株的粘菌素耐药性中起主要作用。我们报道了在保加利亚产生耐粘菌素NDM-1的肺炎克雷伯菌ST11克隆中，IS903样元素截断mgrB。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.