FGF Ligands and Receptors in Osteochondral Tissues of the Temporomandibular Joint in Young and Aging Mice.

IF 2.7 4区医学 Q1 ORTHOPEDICS

CARTILAGE Pub Date : 2024-06-01 Epub Date: 2023-04-26 DOI:10.1177/19476035231163691

Eliane H Dutra, Po-Jung Chen, Zana Kalajzic, Sunil Wadhwa, Marja Hurley, Sumit Yadav

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引用次数: 0

Abstract

Objective: Fibroblast growth factors (FGFs) are a family of 22 proteins and 4 FGF receptors (FGFRs) that are crucial elements for normal development. The contribution of different FGFs and FGFRs for the homeostasis or disease of the cartilage from the mandibular condyle is unknown. Therefore, our goal was to characterize age-related alterations in the protein expression of FGF ligands and FGFRs in the mandibular condyle of mice.

Method: Mandibular condyles of 1-, 6-, 12-, 18-, and 24-month-old C57BL/6J male mice (5 per group) were collected and histologically sectioned. Immunofluorescence for FGFs that have been reported to be relevant for chondrogenesis (FGF2, FGF8, FGF9, FGF18) as well as the activated/phosphorylated FGFRs (pFGFR1, pFGFR3) was carried out.

Results: FGF2 and FGF8 were strongly expressed in the cartilage and subchondral bone of 1-month-old mice, but the expression shifted mainly to the subchondral bone as mice aged. FGF18 and pFGFR3 expression was limited to the cartilage of 1-month-old mice only. Meanwhile, pFGFR1 and FGF9 were mostly limited to the cartilage with a significant increase in expression as mice aged.

Conclusions: Our results indicate FGF2 and FGF8 are important growth factors for mandibular condylar cartilage growth in young mice but with limited role in the cartilage of older mice. In addition, the increased expression of pFGFR1 and FGF9 and the decreased expression of pFGFR3 and FGF18 as mice aged suggest the association of these factors with aging and osteoarthritis of the cartilage of the mandibular condyle.

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年轻和衰老小鼠颞下颌关节骨软骨组织中的FGF配体和受体。

目的：成纤维细胞生长因子（FGFs）是一个由22种蛋白质和4种FGF受体组成的家族，它们是正常发育的关键元件。不同的FGF和FGFR对髁突软骨稳态或疾病的贡献尚不清楚。因此，我们的目标是表征小鼠髁突中FGF配体和FGFR蛋白表达的年龄相关性变化。方法：收集1、6、12、18和24月龄C57BL/6J雄性小鼠（每组5只）的下颌髁，并进行组织切片。对已报道与软骨形成相关的FGF（FGF2、FGF8、FGF9、FGF18）以及活化/磷酸化的FGFR（pFGFR1、pFGFR3）进行免疫荧光。结果：FGF2和FGF8在1个月大的小鼠软骨和软骨下骨中强烈表达，但随着小鼠年龄的增长，其表达主要转移到软骨下骨。FGF18和pFGFR3的表达仅限于1个月大的小鼠的软骨。同时，pFGFR1和FGF9主要局限于软骨，随着小鼠年龄的增长，表达显著增加。结论：FGF2和FGF8是年轻小鼠下颌髁突软骨生长的重要生长因子，但在老年小鼠软骨中的作用有限。此外，随着小鼠年龄的增长，pFGFR1和FGF9的表达增加，而pFGFR3和FGF18的表达减少，这表明这些因素与衰老和下颌髁软骨骨关节炎有关。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.