Sarcocystis cruzi in Egyptian slaughtered cattle (Bos taurus): epidemiology, morphology and molecular description of the findings.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2022-01-01 DOI:10.22099/IJVR.2022.43498.6363

I S Elshahawy, E Mohammed, A Gomaa, M Fawaz

{"title":"Sarcocystis cruzi in Egyptian slaughtered cattle (Bos taurus): epidemiology, morphology and molecular description of the findings.","authors":"I S Elshahawy, E Mohammed, A Gomaa, M Fawaz","doi":"10.22099/IJVR.2022.43498.6363","DOIUrl":null,"url":null,"abstract":"Background: Sarcocystis spp. are one of the most common foodborne tissue cyst-forming coccidia with a public health and veterinary concern.Aims: The existing study aimed to rectify the epidemiological profile of Sarcocystis spp. infection in the cattle carcasses as well as to explore the structure and phylogenetic features of Sarcocystis spp. isolates.Methods: A total of 292 cattle carcasses were checked for the existence of sarcocysts using light microscopy (LM) via muscle squash (MS) and peptic digestion (PD) analysis from January 2020 to December 2020. Individual sarcocysts from different cattle tissues were selected for morphologic characterization and DNA extraction. Each sarcocyst's 18S rDNA gene was amplified, sequenced, and analyzed.Results: Overall, 92.5% (270/292) of cattle tissue samples contained microscopic thin walled sarcocysts and were exclusively found in esophagus by light microscopy. A statistically insignificant relationship exists between the prevalence of infection and age groups, gender of cattle, and the seasonal dynamics (P>0.05). Sarcocysts ultrastructural features were completely discussed. Sequencing of 18S rDNA Sarcocystis gene confirmed S. cruzi (identity 99-100%), which was the first molecular identification of the current isolate in the study region.Conclusion: The current survey initially provides a brief account of knowledge about the epidemiology of Sarcocystis spp. infecting cattle and it is considered a starting point for the development of health awareness and efficient preventive schemes for this zoonotic protozoan parasite.","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"23 4","pages":"337-348"},"PeriodicalIF":0.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0b/ba/ijvr-23-337.PMC9984137.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2022.43498.6363","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Sarcocystis spp. are one of the most common foodborne tissue cyst-forming coccidia with a public health and veterinary concern.

Aims: The existing study aimed to rectify the epidemiological profile of Sarcocystis spp. infection in the cattle carcasses as well as to explore the structure and phylogenetic features of Sarcocystis spp. isolates.

Methods: A total of 292 cattle carcasses were checked for the existence of sarcocysts using light microscopy (LM) via muscle squash (MS) and peptic digestion (PD) analysis from January 2020 to December 2020. Individual sarcocysts from different cattle tissues were selected for morphologic characterization and DNA extraction. Each sarcocyst's 18S rDNA gene was amplified, sequenced, and analyzed.

Results: Overall, 92.5% (270/292) of cattle tissue samples contained microscopic thin walled sarcocysts and were exclusively found in esophagus by light microscopy. A statistically insignificant relationship exists between the prevalence of infection and age groups, gender of cattle, and the seasonal dynamics (P>0.05). Sarcocysts ultrastructural features were completely discussed. Sequencing of 18S rDNA Sarcocystis gene confirmed S. cruzi (identity 99-100%), which was the first molecular identification of the current isolate in the study region.

Conclusion: The current survey initially provides a brief account of knowledge about the epidemiology of Sarcocystis spp. infecting cattle and it is considered a starting point for the development of health awareness and efficient preventive schemes for this zoonotic protozoan parasite.

Abstract Image

查看原文本刊更多论文

埃及屠宰牛的克氏肉囊菌:流行病学、形态学和分子描述。

背景:肉孢子虫是最常见的食源性组织囊肿形成球虫之一，引起了公共卫生和兽医的关注。目的:本研究旨在纠正牛尸体中肌孢子虫感染的流行病学概况，并探讨分离的肌孢子虫的结构和系统发育特征。方法:于2020年1月至2020年12月对292具牛胴体进行肌挤压(MS)和消化消化(PD)光镜检查，检查是否存在肌囊。选取不同牛组织的单个肌囊进行形态学表征和DNA提取。扩增每个结节的18S rDNA基因，测序并分析。结果:总体而言，92.5%(270/292)的牛组织样本含有显微镜下的薄壁结节囊肿，光镜下仅在食道发现。感染率与牛的年龄、性别和季节动态关系不显著(P>0.05)。并对结节囊的超微结构特征进行了全面讨论。18S rDNA肌囊菌基因测序证实了克氏沙门氏菌(同源性99-100%)，这是该研究区目前分离物的首次分子鉴定。结论:目前的调查初步提供了感染牛的肉囊虫的流行病学知识，并被认为是发展对这种人畜共患原生动物寄生虫的健康意识和有效预防方案的起点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.