De Novo Genome Assembly of Auanema Melissensis, a Trioecious Free-Living Nematode.

IF 1.4 4区生物学 Q2 ZOOLOGY

Journal of nematology Pub Date : 2023-02-07 eCollection Date: 2022-02-01 DOI:10.2478/jofnem-2022-0059

Sophie Tandonnet, Maairah Haq, Anisa Turner, Theresa Grana, Panagiota Paganopoulou, Sally Adams, Sandhya Dhawan, Natsumi Kanzaki, Isabelle Nuez, Marie-Anne Félix, André Pires-daSilva

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Abstract

Nematodes of the genus Auanema are interesting models for studying sex determination mechanisms because their populations consist of three sexual morphs (males, females, and hermaphrodites) and produce skewed sex ratios. Here, we introduce a new undescribed species of this genus, Auanema melissensis n. sp., together with its draft nuclear genome. This species is also trioecious and does not cross with the other described species A. rhodensis or A. freiburgensis. Similar to A. freiburgensis, A. melissensis' maternal environment influences the hermaphrodite versus female sex determination of the offspring. The genome of A. melissensis is ~60 Mb, containing 11,040 protein-coding genes and 8.07% of repeat sequences. Using the estimated ancestral chromosomal gene content (Nigon elements), it was possible to identify putative X chromosome scaffolds.

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一种雌雄同株的自由生活线虫 Auanema Melissensis 的全新基因组组装。

Auanema 属线虫是研究性别决定机制的有趣模型，因为它们的种群由三种性态（雄性、雌性和雌雄同体）组成，并产生倾斜的性别比例。在此，我们介绍了该属中一个未被描述的新物种 Auanema melissensis n. sp.及其核基因组草案。该种也是雌雄同株，并且不与其他已描述的种 A. rhodensis 或 A. freiburgensis 杂交。与 A. freiburgensis 相似，A. melissensis 的母源环境也会影响后代的雌雄性别决定。Melissensis 的基因组约为 60 Mb，包含 11,040 个蛋白编码基因和 8.07% 的重复序列。利用估计的祖先染色体基因含量（Nigon元素），可以确定推定的X染色体支架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of nematology 生物-动物学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

14 weeks

期刊介绍： Journal of Nematology is the official technical and scientific communication publication of the Society of Nematologists since 1969. The journal publishes original papers on all aspects of basic, applied, descriptive, theoretical or experimental nematology and adheres to strict peer-review policy. Other categories of papers include invited reviews, research notes, abstracts of papers presented at annual meetings, and special publications as appropriate.