Modified Zhibai Dihuang pill alleviated urinary tract infection induced by extended-spectrum β-lactamase Escherichia coli in rats by regulating biofilm formation.

IF 3.9 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Kaifa Chen, Yongsheng Zhu, Hongwei Su, Hao Jiang, Xin Liu
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Abstract

Context: Zhibai Dihuang pill (ZD), a traditional Chinese medicine nourishes Yin and reduces internal heat, is believed to have therapeutic effects on urinary tract infections (UTIs).

Objective: To explore the effects and mechanism of modified ZD (MZD) on UTI induced by extended-spectrum β-lactamase (ESBLs) Escherichia coli.

Materials and methods: Thirty Sprague-Dawley rats were randomly divided into control, model (0.5 mL 1.5 × 108 CFU/mL ESBLs E. coli), MZD (20 g/kg MZD), LVFX (0.025 g/kg LVFX), and MZD + LVFX groups (20 g/kg MZD + 0.025 g/kg LVFX), n = 6. After 14 days of treatment, serum biochemical indicators, renal function indicators, bladder and renal histopathology, and urine bacterial counts in rats were determined. Additionally, the effects of MZD on ESBLs E. coli biofilm formation and related gene expression were analyzed.

Results: MZD significantly decreased the count of white blood cells (from 13.12 to 9.13), the proportion of neutrophils (from 43.53 to 23.18), C-reactive protein (from 13.21 to 9.71), serum creatinine (from 35.78 to 30.15), and urea nitrogen (from 12.56 to 10.15), relieved the inflammation and fibrosis of bladder and kidney tissues, and reduced the number of bacteria in urine (from 2174 to 559). In addition, MZD inhibited the formation of ESBLs E. coli biofilms (2.04-fold) and decreased the gene expressions of luxS, pfS and ompA (1.41-1.62-fold).

Discussion and conclusion: MZD treated ESBLs E. coli-induced UTI inhibited biofilm formation, providing a theoretical basis for the clinical application of MZD. Further study on the clinical effect of MZD may provide a novel therapy option for UTI.

Abstract Image

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Abstract Image

栀白地黄丸通过调节生物膜的形成,减轻广谱β-内酰胺酶引起的大鼠尿路感染。
背景:知白地黄丸是一种滋阴降内热的中药,具有治疗尿路感染的作用。材料和方法:30只Sprague-Dawley大鼠随机分为对照组、模型组(0.5 毫升1.5 × 108 CFU/mL ESBLs大肠杆菌)、MZD(20 g/kg MZD)、LVFX(0.025 g/kg LVFX)和MZD + LVFX组(20 g/kg MZD+0.025 g/kg LVFX),n = 6.治疗14天后,测定大鼠血清生化指标、肾功能指标、膀胱和肾脏组织病理学以及尿液细菌计数。此外,还分析了MZD对ESBLs大肠杆菌生物膜形成和相关基因表达的影响。结果:MZD显著降低了白细胞计数(从13.12降至9.13)、中性粒细胞比例(从43.53降至23.18)、C反应蛋白(从13.21降至9.71)、血清肌酐(从35.78降至30.15)和尿素氮(从12.56降至10.15),缓解了膀胱和肾组织的炎症和纤维化,并减少了尿液中的细菌数量(从2174降至559)。此外,MZD抑制ESBLs大肠杆菌生物膜的形成(2.04倍),降低luxS、pfS和ompA的基因表达(1.41-1.62倍)。进一步研究MZD的临床疗效可能为UTI提供一种新的治疗选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Pharmaceutical Biology
Pharmaceutical Biology 医学-药学
CiteScore
6.70
自引率
2.60%
发文量
191
审稿时长
1 months
期刊介绍: Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.
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