Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas.

IF 1.5 4区 生物学 Q4 MYCOLOGY
Mycoscience Pub Date : 2021-03-20 eCollection Date: 2021-01-01 DOI:10.47371/mycosci.2020.12.001
Keisuke Obase, Satoshi Yamanaka, Akihiko Kinoshita, Yutaka Tamai, Takashi Yamanaka
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Abstract

Pure cultures of Tuber were isolated from ectomycorrhizal root tips in Abies sachalinensis plantations in Hokkaido, Japan. Their phylogenetic relationships as well as vegetative hyphal characteristics on culture media were reported. Phylogenetic analysis based on the internal transcribed spacer within ribosomal DNA settled well-supported eight lineages within Puberulum, Latisporum, and Maculatum clades in Tuber. Three and one lineages were grouped with undescribed species of Puberulum clade in Japan and that of the Latisporum group in China, respectively. Two lineages were closely associated to but distinct from an undescribed species of Puberulum clade in Japan. One lineage did not group with any sequences in the International Nucleotide Sequence Database (INSD), proposing a new taxon in the Latisporum group. One lineage was grouped with T. foetidum in Maculatum clade. All strains in each lineage displayed yellowish white, thin, filamentous colonies on Melin-Norkrans agar medium. Various differences in morphological characteristics of hyphae on pure cultures of various strains were noted, but they were frequently uncommon among strains of the same taxa. Isolation from ectomycorrhizal root tips can be among the effective ways to acquire pure cultures of Tuber strains.

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从外生菌根中分离出的块茎物种的系统发育位置和文化特征。
从日本北海道Abies sachalinensis种植园的外生菌根尖中分离出了块根的纯培养物。报告了它们的系统发育关系以及在培养基上的无性菌丝特征。基于核糖体 DNA 内部转录间隔的系统发育分析确定了 Tuber 中 Puberulum、Latisporum 和 Maculatum 支系中的八个系。日本的 Puberulum 支系和中国的 Latisporum 支系中分别有 3 个和 1 个世系与未描述的物种同属一个支系。两个品系与日本未描述的 Puberulum 支系中的一个物种密切相关,但又截然不同。一个品系与国际核苷酸序列数据库(INSD)中的任何序列都没有关联,因此被认为是 Latisporum 群中的一个新类群。一个品系与 T. foetidum 一起归入 Maculatum 支系。每个品系的所有菌株在 Melin-Norkrans 琼脂培养基上都显示出黄白色、细丝状菌落。在不同菌株的纯培养物上,菌丝的形态特征存在各种差异,但这些差异在同一类群的菌株中并不常见。从外生菌根尖分离是获得块根菌株纯培养物的有效方法之一。
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来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
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