Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson's Disease.

IF 1.6 4区生物学 Q4 CELL BIOLOGY

Acta Histochemica Et Cytochemica Pub Date : 2023-04-25 DOI:10.1267/ahc.22-00109

Haruka Tsubaki, Daijiro Yanagisawa, Yusuke Kageyama, Zulzikry Hafiz Abu Baker, Ken-Ichi Mukaisho, Ikuo Tooyama

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Abstract

Mitochondrial ferritin (FtMt) is an endogenous iron-storage protein localized in the mitochondria. FtMt is mainly observed in restricted tissues, such as those in the testis, islets of Langerhans, and brain. Further, it may protect cells from oxidative stress in neurodegenerative diseases, including Alzheimer's disease and progressive supranuclear palsy. However, the role of FtMt in Parkinson's disease (PD) remains unclear. Therefore, the current study investigated the localization and expression level of FtMt in the midbrain of patients with PD and healthy controls using immunohistochemical techniques. FtMt immunoreactivity was mainly detected in dopaminergic neurons in the substantia nigra pars compacta (SNc) in both healthy controls and patients with PD. In addition, FtMt-positive particles were observed outside the dopaminergic neurons in patients with PD. Based on a quantitative comparison, patients with PD had a significantly upregulated FtMt immunoreactivity in dopaminergic neurons than healthy controls. Our result might be helpful in future studies on the role of FtMt in PD.

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帕金森病患者中脑线粒体铁蛋白的免疫组化分析

线粒体铁蛋白(FtMt)是一种内源性的铁储存蛋白，定位于线粒体。FtMt主要见于受限组织，如睾丸、朗格汉斯岛和大脑。此外，它可以保护细胞免受神经退行性疾病的氧化应激，包括阿尔茨海默病和进行性核上性麻痹。然而，FtMt在帕金森病(PD)中的作用尚不清楚。因此，本研究利用免疫组织化学技术研究了PD患者和健康对照中脑FtMt的定位和表达水平。FtMt免疫反应性主要在健康对照和PD患者的黑质致密部(SNc)多巴胺能神经元中检测到。此外，PD患者多巴胺能神经元外可见ftmt阳性颗粒。定量比较发现，PD患者多巴胺能神经元的FtMt免疫反应性明显高于健康对照组。我们的结果可能有助于进一步研究FtMt在PD中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Histochemica Et Cytochemica 生物-细胞生物学

CiteScore

3.50

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Histochemica et Cytochemica is the official online journal of the Japan Society of Histochemistry and Cytochemistry. It is intended primarily for rapid publication of concise, original articles in the fields of histochemistry and cytochemistry. Manuscripts oriented towards methodological subjects that contain significant technical advances in these fields are also welcome. Manuscripts in English are accepted from investigators in any country, whether or not they are members of the Japan Society of Histochemistry and Cytochemistry. Manuscripts should be original work that has not been previously published and is not being considered for publication elsewhere, with the exception of abstracts. Manuscripts with essentially the same content as a paper that has been published or accepted, or is under consideration for publication, will not be considered. All submitted papers will be peer-reviewed by at least two referees selected by an appropriate Associate Editor. Acceptance is based on scientific significance, originality, and clarity. When required, a revised manuscript should be submitted within 3 months, otherwise it will be considered to be a new submission. The Editor-in-Chief will make all final decisions regarding acceptance.