Effect of intravesical tarantula cubensis extract (Theranekron) on inflammation in an interstitial cystitis rat model

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

LUTS: Lower Urinary Tract Symptoms Pub Date : 2022-12-07 DOI:10.1111/luts.12470

Abdullah Akdağ, Çiğdem Yenisey, Tuğrul E. Epikmen, Erhan Ateş

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引用次数: 0

Abstract

Objectives

To reveal the histopathological and immunological outcomes of intravesical treatment with tarantula cubensis extract (TCE) in a rat model of interstitial cystitis.

Methods

A total of 30 female Wistar albino rats were divided into three groups: group 1 (control group), group 2 (disease group), and group 3 (treatment group). The rat model of interstitial cystitis was created by biweekly intraperitoneal administration of cyclophosphamide (CYP). In group 3, TCE (a venom extracted from a brown spider known as tarantula cubensis) was administered intravesically after the model had been created. Urothelial degeneration, necrosis, ulcer, bleeding, edema, inflammation and mast cell count, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), myeloperoxidase (MPO), and hydroxyproline parameters were evaluated. Statistical analysis was performed using one-way analysis of variance, chi-square tests, and Kruskal–Wallis tests.

Results

All parameters were found to be lower in the rats in group 1 than in the other groups, and IL-6 and MPO values were found to be higher in group 2 (p < .001). The mean TNF-alpha value was highest in group 2 (p = .078). No difference was found between all groups regarding ulcer (p = .087). Urothelial degeneration, necrosis, edema, inflammation, hemorrhage and fibroblast proliferations, and hydroxyproline values were higher in group 3 (p < .001).

Conclusions

Intravesical TCE instillation produces an anti-inflammatory effect by reducing the levels of inflammatory parameters such as IL-6, TNF-alpha, and MPO in bladder tissue. It also accelerates tissue healing by increasing hydroxyproline and fibroblast proliferation.

查看原文本刊更多论文

膀胱内狼蛛提取物(Theranekron)对间质性膀胱炎大鼠模型炎症的影响

目的观察狼蛛提取物(TCE)膀胱内治疗间质性膀胱炎模型大鼠的组织病理学和免疫学结果。方法将30只雌性Wistar白化大鼠随机分为3组:1组(对照组)、2组(疾病组)、3组(治疗组)。采用两周腹腔注射环磷酰胺(CYP)建立间质性膀胱炎大鼠模型。在第三组，TCE(一种从被称为狼蛛的棕色蜘蛛中提取的毒液)在模型创建后被静脉注射。评估尿路上皮变性、坏死、溃疡、出血、水肿、炎症和肥大细胞计数、白细胞介素-6 (IL-6)、肿瘤坏死因子- α (tnf - α)、髓过氧化物酶(MPO)和羟脯氨酸参数。统计分析采用单因素方差分析、卡方检验和Kruskal-Wallis检验。结果1组大鼠各项指标均低于其他各组，2组大鼠IL-6、MPO值均高于其他各组(p < .001)。平均tnf - α值以2组最高(p = 0.078)。溃疡方面各组间无差异(p = 0.087)。3组尿路上皮变性、坏死、水肿、炎症、出血、成纤维细胞增生、羟脯氨酸值增高(p < .001)。结论膀胱内灌注TCE可降低膀胱组织炎症参数IL-6、tnf - α和MPO水平，具有抗炎作用。它还通过增加羟脯氨酸和成纤维细胞增殖来加速组织愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

LUTS: Lower Urinary Tract Symptoms UROLOGY & NEPHROLOGY-

CiteScore

3.00

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： LUTS is designed for the timely communication of peer-reviewed studies which provides new clinical and basic science information to physicians and researchers in the field of neurourology, urodynamics and urogynecology. Contributions are reviewed and selected by a group of distinguished referees from around the world, some of whom constitute the journal''s Editorial Board. The journal covers both basic and clinical research on lower urinary tract dysfunctions (LUTD), such as overactive bladder (OAB), detrusor underactivity, benign prostatic hyperplasia (BPH), bladder outlet obstruction (BOO), urinary incontinence, pelvic organ prolapse (POP), painful bladder syndrome (PBS), as well as on other relevant conditions. Case reports are published only if new findings are provided. LUTS is an official journal of the Japanese Continence Society, the Korean Continence Society, and the Taiwanese Continence Society. Submission of papers from all countries are welcome. LUTS has been accepted into Science Citation Index Expanded (SCIE) with a 2011 Impact Factor.