Bacillus velezensis S141, a soybean growth-promoting bacterium, hydrolyzes isoflavone glycosides into aglycones.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Takahiko Kondo, Surachat Sibponkrung, Ken-Yu Hironao, Panlada Tittabutr, Nantakorn Boonkerd, Shu Ishikawa, Hitoshi Ashida, Neung Teaumroong, Ken-Ichi Yoshida
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引用次数: 1

Abstract

Bacillus velezensis S141, a plant growth-promoting rhizobacteria (PGPR), was isolated from a soybean field in Thailand. Previous studies demonstrated that S141 enhanced soybean growth, stimulating nodulation for symbiotic nitrogen fixation with soybean root nodule bacteria, including Bradyrhizobium diazoefficience USDA110. Isoflavone glycosides are produced in soybean roots and hydrolyzed into their aglycones, triggering nodulation. This study revealed that S141 efficiently hydrolyzed two isoflavone glycosides in soybean roots (daidzin and genistin) to their aglycones (daidzein and genistein, respectively). However, S141, Bacillus subtilis 168, NCIB3610, and B. velezensis FZB42 hydrolyzed isoflavone glucosides into aglycones. A BLASTp search suggested that S141 and the other three strains shared four genes encoding β-glucosidases corresponding to bglA, bglC, bglH, and gmuD in B. subtilis 168. The gene inactivation analysis of B. subtilis 168 revealed that bglC encoded the major β-glucosidase, contributing about half of the total activity to hydrolyze isoflavone glycosides and that bglA, bglH, and gmuD, all barely committed to the hydrolysis of isoflavone glycosides. Thus, an unknown β-glucosidase exists, and our genetic knowledge of β-glucosidases was insufficient to evaluate the ability to hydrolyze isoflavone glycosides. Nevertheless, S141 could predominate in the soybean rhizosphere, releasing isoflavone aglycones to enhance soybean nodulation.

velezensis S141是一种促进大豆生长的细菌,它能将异黄酮苷水解成苷元。
从泰国大豆田分离到一株促进植物生长的根瘤菌velezensis S141。先前的研究表明,S141促进了大豆的生长,促进了与大豆根瘤细菌(包括重氮效率慢生根瘤菌USDA110)共生固氮的结瘤。大豆根部产生异黄酮苷,并水解成其苷元,引发结瘤。本研究发现,S141能有效地将大豆根中的两种异黄酮苷类化合物(大豆苷元和染料木素)水解为它们的苷元(大豆苷元和染料木素)。而S141、枯草芽孢杆菌168、NCIB3610和B. velezensis FZB42能将异黄酮苷水解成苷元。BLASTp检索结果表明,S141与其他3株菌株共有4个基因编码β-葡萄糖苷酶,分别对应枯草芽孢杆菌168中的bglA、bglC、bglH和gmuD。对枯草芽孢杆菌168的基因失活分析表明,bglC编码了主要的β-葡萄糖苷酶,对异黄酮苷的水解贡献了约一半的总活性,而bglA、bglH和gmuD几乎不参与异黄酮苷的水解。因此,存在一种未知的β-葡萄糖苷酶,我们对β-葡萄糖苷酶的遗传知识不足以评估其水解异黄酮苷的能力。然而,S141在大豆根际中占优势,释放异黄酮苷元促进大豆结瘤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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