Applying the Cytocentric Principles to Regenerative Medicine for Reproducibility.

IF 2.3 Q4 CELL & TISSUE ENGINEERING

Current Stem Cell Reports Pub Date : 2022-01-01 DOI:10.1007/s40778-022-00219-8

Alicia D Henn, Kunal Mitra, Joshua Hunsberger, Xiuzhi Susan Sun, Mark Nardone, Ramon Montero, Sita Somara, Gary Green, Alan Blanchard, Yu Shrike Zhang, Carl G Simon, Randy Yerden

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Abstract

Purpose of review: Cell and tissue products do not just reflect their present conditions; they are the culmination of all they have encountered over time. Currently, routine cell culture practices subject cell and tissue products to highly variable and non-physiologic conditions. This article defines five cytocentric principles that place the conditions for cells at the core of what we do for better reproducibility in Regenerative Medicine.

Recent findings: There is a rising awareness of the cell environment as a neglected, but critical variable. Recent publications have called for controlling culture conditions for better, more reproducible cell products.

Summary: Every industry has basic quality principles for reproducibility. Cytocentric principles focus on the fundamental needs of cells: protection from contamination, physiologic simulation, and full-time conditions for cultures that are optimal, individualized, and dynamic. Here, we outline the physiologic needs, the technologies, the education, and the regulatory support for the cytocentric principles in regenerative medicine.

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细胞中心原理在再生医学中的应用。

综述目的:细胞和组织产品不只是反映其现状;它们是它们随着时间的推移所遇到的一切的顶点。目前，常规细胞培养实践将细胞和组织产物置于高度可变的非生理性条件下。本文定义了五个细胞中心原则，这些原则将细胞条件置于我们在再生医学中为更好的可重复性所做的工作的核心。最近的发现:人们越来越意识到细胞环境是一个被忽视的关键变量。最近的出版物呼吁控制培养条件，以获得更好、更可再生的细胞产品。每个行业都有重复性的基本质量原则。细胞中心原理关注细胞的基本需求:免受污染的保护，生理模拟，以及最佳、个性化和动态培养的全职条件。在此，我们概述了再生医学中细胞中心原理的生理需求、技术、教育和调控支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Stem Cell Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： The goal of this journal is to publish cutting-edge reviews on subjects pertinent to all aspects of stem cell research, therapy, ethics, commercialization, and policy. We aim to provide incisive, insightful, and balanced contributions from leading experts in each relevant domain that will be of immediate interest to a wide readership of clinicians, basic scientists, and translational investigators. We accomplish this aim by appointing major authorities to serve as Section Editors in key subject areas across the discipline. Section Editors select topics to be reviewed by leading experts who emphasize recent developments and highlight important papers published over the past year on their topics, in a crisp and readable format. We also provide commentaries from well-known figures in the field, and an Editorial Board of internationally diverse members suggests topics of special interest to their country/region and ensures that topics are current and include emerging research.