Effect of copper, arsenic and nickel on pyrite-based autotrophic denitrification

IF 3.1 4区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biodegradation Pub Date : 2023-04-28 DOI:10.1007/s10532-023-10027-4

Maria F. Carboni, Sonia Arriaga, Piet N. L. Lens

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Abstract

Pyritic minerals generally occur in nature together with other trace metals as impurities, that can be released during the ore oxidation. To investigate the role of such impurities, the presence of copper (Cu(II)), arsenic (As(III)) and nickel (Ni(II)) during pyrite mediated autotrophic denitrification has been explored in this study at 30 °C with a specialized microbial community of denitrifiers as inoculum. The three metal(loid)s were supplemented at an initial concentration of 2, 5, and 7.5 ppm and only Cu(II) had an inhibitory effect on the autotrophic denitrification. The presence of As(III) and Ni(II) enhanced the nitrate removal efficiency with autotrophic denitrification rates between 3.3 [7.5 ppm As(III)] and 1.6 [7.5 ppm Ni(II)] times faster than the experiment without any metal(loid) supplementation. The Cu(II) batches, instead, decreased the denitrification kinetics with 16, 40 and 28% compared to the no-metal(loid) control for the 2, 5 and 7.5 ppm incubations, respectively. The kinetic study revealed that autotrophic denitrification with pyrite as electron donor, also with Cu(II) and Ni(II) additions, fits better a zero-order model, while the As(III) incubation followed first-order kinetic. The investigation of the extracellular polymeric substances content and composition showed more abundance of proteins, fulvic and humic acids in the metal(loid) exposed biomass.

Graphical Abstract

Abstract Image

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铜、砷和镍对黄铁矿自养反硝化作用的影响。

黄铁矿一般与其他痕量金属一起作为杂质存在于自然界中，在矿石氧化过程中会释放出来。为了研究这些杂质的作用，本研究以专门的反硝化微生物群落为接种物，在 30 ° C 条件下，探讨了黄铁矿介导的自养反硝化过程中铜（Cu(II)）、砷（As(III)）和镍（Ni(II)）的存在。三种金属（loid）的初始添加浓度分别为百万分之 2、5 和 7.5，其中只有铜（II）对自养反硝化作用有抑制作用。As(III)和 Ni(II)的存在提高了硝酸盐的去除效率，自养反硝化速度比未添加任何金属（loid）的实验快 3.3 [7.5 ppm As(III)]和 1.6 [7.5 ppm Ni(II)]倍。相反，在 2、5 和 7.5 ppm 的培养条件下，与无金属（loid）对照组相比，Cu（II）批次的反硝化动力学分别降低了 16%、40% 和 28%。动力学研究表明，以黄铁矿为电子供体的自养反硝化作用，以及添加铜（II）和镍（II）的自养反硝化作用更适合零阶模型，而砷（III）培养则遵循一阶动力学。对细胞外高分子物质含量和组成的调查显示，暴露于金属（loid）的生物质中蛋白质、富里酸和腐殖酸含量更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biodegradation 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Biodegradation publishes papers, reviews and mini-reviews on the biotransformation, mineralization, detoxification, recycling, amelioration or treatment of chemicals or waste materials by naturally-occurring microbial strains, microbial associations, or recombinant organisms. Coverage spans a range of topics, including Biochemistry of biodegradative pathways; Genetics of biodegradative organisms and development of recombinant biodegrading organisms; Molecular biology-based studies of biodegradative microbial communities; Enhancement of naturally-occurring biodegradative properties and activities. Also featured are novel applications of biodegradation and biotransformation technology, to soil, water, sewage, heavy metals and radionuclides, organohalogens, high-COD wastes, straight-, branched-chain and aromatic hydrocarbons; Coverage extends to design and scale-up of laboratory processes and bioreactor systems. Also offered are papers on economic and legal aspects of biological treatment of waste.