LncRNA PNKY Is Upregulated in Breast Cancer and Promotes Cell Proliferation and EMT in Breast Cancer Cells.

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Non-Coding RNA Pub Date : 2023-04-06 DOI:10.3390/ncrna9020025

Forough Hakiminia, Firooz Jannat Alipoor, Mostafa Keshavarz, Malek Hossein Asadi

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引用次数: 0

Abstract

Long non-coding RNAs (lncRNAs) are known to be important regulators in different cellular processes and are implicated in various human diseases. Recently, lncRNA PNKY has been found to be involved in pluripotency and differentiation of embryonic and postnatal neural stem cells (NSCs); however, its expression and function in cancer cells is still unclear. In the present study, we observed the expression of PNKY in various cancer tissues, including brain, breast, colorectal, and prostate cancers. In particular, we demonstrated that lncRNA PNKY was significantly upregulated in breast tumors, especially high-grade tumors. Knock down experiments indicated that the suppression of PNKY in breast cancer cells could restrict their proliferation by promoting apoptosis, senescence, and cell cycle disruption. Moreover, the results demonstrated that PNKY may play a crucial role in the cell migration of breast cancer cells. We further found that PNKY may trigger EMT in breast cancer cells by upregulating miR-150 and restricting the expression of Zeb1 and Snail. This study is the first to provide new evidence on the expression and biological function of PNKY in cancer cells and its potential contribution to tumor growth and metastasis.

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LncRNA PNKY在乳腺癌中表达上调，促进乳腺癌细胞增殖和EMT

已知长链非编码rna (lncRNAs)在不同的细胞过程中是重要的调节因子，并与各种人类疾病有关。最近，lncRNA PNKY被发现参与胚胎和出生后神经干细胞(NSCs)的多能性和分化;然而，其在癌细胞中的表达和功能尚不清楚。在本研究中，我们观察了PNKY在各种癌症组织中的表达，包括脑癌、乳腺癌、结直肠癌和前列腺癌。特别是，我们证明lncRNA PNKY在乳腺肿瘤，特别是高级别肿瘤中显著上调。敲低实验表明，PNKY在乳腺癌细胞中的抑制可以通过促进细胞凋亡、衰老和细胞周期破坏来限制其增殖。此外，结果表明PNKY可能在乳腺癌细胞的细胞迁移中起关键作用。我们进一步发现，PNKY可能通过上调miR-150并抑制Zeb1和Snail的表达而触发乳腺癌细胞的EMT。本研究首次为PNKY在肿瘤细胞中的表达、生物学功能及其在肿瘤生长和转移中的潜在作用提供了新的证据。

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.