{"title":"A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample.","authors":"Yusuke Okazaki, Tuyen Thi Nguyen, Arisa Nishihara, Hisashi Endo, Hiroyuki Ogata, Shin-Ichi Nakano, Hideyuki Tamaki","doi":"10.1264/jsme2.ME22102","DOIUrl":null,"url":null,"abstract":"<p><p>We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10-15 kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50 ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.</p>","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"38 1","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037101/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbes and Environments","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1264/jsme2.ME22102","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10-15 kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50 ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.
期刊介绍：
Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.
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