In vitro investigation of the antibacterial and anti-inflammatory effects of LED irradiation.

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Periodontal and Implant Science Pub Date : 2023-04-01 DOI:10.5051/jpis.2200920046

Jungwon Lee, Hyun-Yong Song, Sun-Hee Ahn, Woosub Song, Yang-Jo Seol, Yong-Moo Lee, Ki-Tae Koo

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引用次数: 2

Abstract

Purpose: This study aimed to investigate the proper wavelengths for safe levels of light-emitting diode (LED) irradiation with bactericidal and photobiomodulation effects in vitro.

Methods: Cell viability tests of fibroblasts and osteoblasts after LED irradiation at 470, 525, 590, 630, and 850 nm were performed using the thiazolyl blue tetrazolium bromide assay. The bactericidal effect of 470-nm LED irradiation was analyzed with Streptococcus gordonii, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Tannerella forsythia. Levels of nitric oxide, a proinflammatory mediator, were measured to identify the anti-inflammatory effect of LED irradiation on lipopolysaccharide-stimulated inflammation in RAW 264.7 macrophages.

Results: LED irradiation at wavelengths of 470, 525, 590, 630, and 850 nm showed no cytotoxic effect on fibroblasts and osteoblasts. LED irradiation at 630 and 850 nm led to fibroblast proliferation compared to no LED irradiation. LED irradiation at 470 nm resulted in bactericidal effects on S. gordonii, A. actinomycetemcomitans, F. nucleatum, P. gingivalis, and T. forsythia. Lipopolysaccharide (LPS)-induced RAW 264.7 inflammation was reduced by irradiation with 525-nm LED before LPS treatment and irradiation with 630-nm LED after LPS treatment; however, the effects were limited.

Conclusions: LED irradiation at 470 nm showed bactericidal effects, while LED irradiation at 525 and 630 nm showed preventive and treatment effects on LPS-induced RAW 264.7 inflammation. The application of LED irradiation has potential as an adjuvant in periodontal therapy, although further investigations should be performed in vivo.

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LED辐照抗菌抗炎作用的体外研究。

目的:探讨具有体外杀菌和光生物调节作用的发光二极管(LED)的安全照射波长。方法:采用噻唑蓝溴化四氮唑法检测LED辐照470、525、590、630和850 nm后成纤维细胞和成骨细胞的细胞活力。研究了470 nm LED辐照对戈登链球菌、放线菌聚集菌、核梭杆菌、牙龈卟啉单胞菌和连翘单宁菌的杀菌效果。我们测量了促炎介质一氧化氮的水平，以确定LED照射对RAW 264.7巨噬细胞中脂多糖刺激的炎症的抗炎作用。结果:470、525、590、630和850 nm波长的LED照射对成纤维细胞和成骨细胞无细胞毒性作用。与未照射相比，630 nm和850 nm LED照射可导致成纤维细胞增殖。470 nm的LED照射对哥氏葡萄球菌、放线菌、核仁葡萄球菌、牙龈葡萄球菌和连翘葡萄球菌均有杀灭作用。脂多糖(LPS)诱导的RAW 264.7炎症在LPS处理前525 nm的LED照射和LPS处理后630 nm的LED照射均可降低;然而，效果是有限的。结论:470 nm LED照射具有杀菌作用，525和630 nm LED照射对lps诱导的RAW 264.7炎症具有预防和治疗作用。LED照射在牙周治疗中的辅助应用具有潜力，但还需在体内进行进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Periodontal and Implant Science DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.30%

发文量

期刊介绍： Journal of Periodontal & Implant Science (JPIS) is a peer-reviewed and open-access journal providing up-to-date information relevant to professionalism of periodontology and dental implantology. JPIS is dedicated to global and extensive publication which includes evidence-based original articles, and fundamental reviews in order to cover a variety of interests in the field of periodontal as well as implant science.