Yan M Crane, Charles F Crane, Sue E Cambron, Lucy J Springmeyer, Brandon J Schemerhorn
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引用次数: 0
Abstract
Like other cecidomyiid Diptera, Hessian fly has stable S chromosomes and dispensable E chromosomes that are retained only in the germ line. Amplified fragment length polymorphisms (AFLP), suppressive subtractive hybridization (SSH), fluorescent in-situ hybridization (FISH), and sequencing were used to investigate similarities and differences between S and E chromosomes. More than 99.9% of AFLP bands were identical between separated ovary and somatic tissue, but one band was unique to ovary and resembled Worf, a non-LTR retrotransposon. Arrayed clones, derived by SSH of somatic from ovarian DNA, showed no clones that were unique to ovary. FISH with BAC clones revealed a diagnostic banding pattern of BAC positions on both autosomes and both sex chromosomes, and each E chromosome shared a pattern with one of the S chromosomes. Sequencing analysis showed that E chromosomes are nearly identical to S chromosomes, since no sequence could be confirmed to belong only to E chromosomes. There were a few questionably E-specific sequences that are candidates for further investigation. Thus, the E chromosomes appear to be derived from S chromosomes by the acquisition or conversion of sequences that produce the negatively heteropycnotic region around the centromere.
与其他头足目双翅目昆虫一样,黑刺蝇具有稳定的 S 染色体和仅保留在种系中的可有可无的 E 染色体。研究人员利用扩增片段长度多态性(AFLP)、抑制性减数杂交(SSH)、荧光原位杂交(FISH)和测序技术研究了S染色体和E染色体的异同。在分离的卵巢和体细胞组织中,99.9%以上的 AFLP 带是相同的,但有一条带是卵巢特有的,类似于非 LTR 逆转录质子 Worf。通过对体细胞 DNA 和卵巢 DNA 进行 SSH 分析得出的阵列克隆显示,没有卵巢特有的克隆。用 BAC 克隆进行的 FISH 显示,常染色体和性染色体上的 BAC 位置都有诊断性的带状模式,每条 E 染色体都与其中一条 S 染色体共享一种模式。测序分析表明,E 染色体与 S 染色体几乎完全相同,因为没有序列可以确认只属于 E 染色体。有一些疑似 E 染色体特异性序列有待进一步研究。因此,E 染色体似乎是通过获得或转换产生中心粒周围负异位区的序列而从 S 染色体衍生而来的。
期刊介绍:
Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to:
· Chromosomes and their linkage to diseases;
· Chromosome organization within the nucleus;
· Chromatin biology (transcription, non-coding RNA, etc);
· Chromosome structure, function and mechanics;
· Chromosome and DNA repair;
· Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting,
dosage compensation, sex determination, chromosome remodeling);
· Architectural/epigenomic organization of the genome;
· Functional annotation of the genome;
· Functional and comparative genomics in plants and animals;
· Karyology studies that help resolve difficult taxonomic problems or that provide
clues to fundamental mechanisms of genome and karyotype evolution in plants and animals;
· Mitosis and Meiosis;
· Cancer cytogenomics.