Increased circulating TGF-β1 is associated with impairment in NK cell effector functions in metastatic melanoma patients.

IF 1.8 4区生物学 Q4 CELL BIOLOGY

Growth factors Pub Date : 2022-11-01 DOI:10.1080/08977194.2022.2124915

Katarina Mirjačić Martinović, Ana Vuletić, Emina Mališić, Tatjana Srdić-Rajić, Nevena Tišma Miletić, Nada Babović, Vladimir Jurišić

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引用次数: 7

Abstract

Transforming growth factor beta (TGF-β) plays a complex role in carcinogenesis. In 30 melanoma patients and 20 healthy controls (HC) we analysed functional and phenotypic characteristics of NK cells by Flow cytometry, gene expression of TGF-β1 in peripheral blood mononuclear cells by qPCR and serum and supernatant level of free TGF-β1 by ELISA. Melanoma patients had significantly higher serum level of circulatingTGF-β1 compared to HC, especially those with metastasis into the central nervous system (subclass M1d) and high LDH serum values. Melanoma patients compared to HC had significantly higher level of TGF-β1 gene in PBMC. TGF-β1 serum values negatively correlate with NK cell activity analysed by CD107a (degranulation marker), IFN-γ, NKG2D, and NKp46 in patients. Study shows the association of high level of TGF-β1 with NK cell inhibition in patients represents the main mechanism of tumour immune evasion. Targeting TGF-β may become an important cancer treatment for improving antitumor immunity.

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在转移性黑色素瘤患者中，循环TGF-β1升高与NK细胞效应物功能受损相关。

转化生长因子β (TGF-β)在癌变过程中发挥着复杂的作用。用流式细胞术分析30例黑色素瘤患者和20例健康对照(HC) NK细胞的功能和表型特征，用qPCR分析外周血单个核细胞TGF-β1基因表达，ELISA分析血清和上清游离TGF-β1水平。黑色素瘤患者血清循环tgf -β1水平明显高于HC，特别是转移到中枢神经系统(M1d亚类)和高LDH血清值的黑色素瘤患者。黑色素瘤患者PBMC中TGF-β1基因水平明显高于HC患者。通过CD107a(脱颗粒标志物)、IFN-γ、NKG2D和NKp46分析患者血清TGF-β1值与NK细胞活性呈负相关。研究表明，患者体内高水平TGF-β1与NK细胞抑制相关是肿瘤免疫逃逸的主要机制。靶向TGF-β可能成为提高抗肿瘤免疫的重要肿瘤治疗手段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications