Dose Dependent Effects of Aqueous Extract of Garcinia cambogia Desr. Against Letrozole Induced Polycystic Ovarian Syndrome in Female Adult Rats With Possible Mechanisms Exploration.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-04-01 DOI:10.1177/15593258231169381

Saba Rana, Liaqat Hussain, Uzma Saleem, Muhammad Asif, Arslan H Lodhi, Muhammad Q Barkat, Muhammad Riaz, Alina Jamil

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引用次数: 3

Abstract

Background: Polycystic ovarian syndrome (PCOS) is an endocrine metabolic disorder of women.

Purpose: This study aimed to explore the potential of aqueous extract of Garcinia cambogia Desr. (AEGC) in PCOS.

Methodology: The HPLC was used to determine the phytoconstituents present in Garcinia cambogia. Thirty adult female albino rats were divided into 6 groups: Normal control (NC) disease Control (PCOS; letrozole 1 mg/kg), plant extract (AEGC 100, 300, 500 mg/kg) and standard (metformin; 20 mg/kg). Disease was confirmed by vaginal smear cytology. After 10 weeks, animals were euthanized, ovaries dissected for histopathology, blood collected for hormonal and biochemical analysis.

Results: HPLC analysis showed the presence of phenolic contents; chlorogenic acid, gallic acid, coumaric acid while flavonoid contents were quercetin, kaempferol, and rutin. After treatment, there was dose dependent reduction of weight, ovarian cysts, improvement of follicle growth. DPPH radical scavenging percentage was 67.89%. Hormonal analysis showed a significant improvement (P < .05) in follicle stimulating hormone (FSH), estrogen, and progesterone while a reduction in testosterone, luteinizing hormone (LH) and insulin level. Antioxidant enzymatic markers were significantly (P < .05) increased. Lipid profile and LFTs were also improved.

Conclusions: The study validated the potential of Garcinia cambogia in the management of PCOS.

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黄藤水提物的剂量依赖性研究。来曲唑诱导雌性成年大鼠多囊卵巢综合征的作用机制探讨。

背景:多囊卵巢综合征(PCOS)是一种女性内分泌代谢性疾病。目的:探讨黄藤水提物的开发潜力。(AEGC)在PCOS。方法:采用高效液相色谱法测定藤黄果中的植物成分。将30只成年雌性白化大鼠分为6组:正常对照(NC)疾病对照(PCOS);来曲唑1mg /kg)、植物提取物(AEGC 100mg /kg、300mg /kg、500mg /kg)和标准品(二甲双胍;20毫克/公斤)。经阴道涂片细胞学检查确诊。10周后，对动物实施安乐死，解剖卵巢进行组织病理学检查，采集血液进行激素和生化分析。结果:HPLC分析显示黄芪中存在酚类成分;绿原酸、没食子酸、香豆酸，类黄酮含量为槲皮素、山奈酚和芦丁。治疗后，体重减轻，卵巢囊肿，卵泡生长改善，均呈剂量依赖性。DPPH自由基清除率为67.89%。激素分析显示，促卵泡激素(FSH)、雌激素和黄体酮水平显著改善(P < 0.05)，睾酮、黄体生成素(LH)和胰岛素水平降低。抗氧化酶指标显著升高(P < 0.05)。血脂和LFTs也有所改善。结论:该研究验证了藤黄果在PCOS治疗中的潜力。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464