Administration of Exendin-4 but not CCK alters lick responses and trial initiation to sucrose and intralipid during brief-access tests.

IF 2.8 4区 心理学 Q1 BEHAVIORAL SCIENCES
Yada Treesukosol, Timothy H Moran
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引用次数: 0

Abstract

Administration of cholecystokinin (CCK) or the glucagon-like peptide 1 (GLP-1) receptor agonist Exendin-4 (Ex-4) reduces food intake. Findings in the literature suggest CCK reduces intake primarily as a satiety signal whereas GLP-1 may play a role in both satiety and reward-related feeding signals. Compounds that humans describe as âsweetâ and âfattyâ are palatable yet are signaled via separate transduction pathways. Here, unconditioned lick responses to sucrose and intralipid were measured in a brief-access lick procedure in food-restricted male rats in response to i.p. administration of Ex-4 (3 h before test), CCK (30 min before test), or a combination of both. The current experimental design measures lick responses to water and varying concentrations of both sucrose (0.03, 0.1, and 0.5 M) and intralipid (0.2%, 2%, and 20%) during 10-s trials across a 30-min single test session. This design minimized postingestive influences. Compared with saline-injected controls, CCK (1.0, 3.0, or 6.0 µg/kg) did not change lick responses to sucrose or intralipid. Number of trials initiated and lick responses to both sucrose and intralipid were reduced in rats injected with 3.0 µg/kg, but not 1.0 µg/kg Ex-4. The supplement of CCK did not alter lick responses or trials initiated compared with Ex-4 administration alone. These findings support a role for GLP-1 but not CCK in the oral responsiveness to palatable stimuli. Furthermore, Ex-4-induced reductions were observed for both sucrose and intralipid, compounds representing âsweetâ and âfat,â respectively.

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施用 Exendin-4 而非 CCK 会改变短暂进入试验中对蔗糖和内脂的舔食反应和试验启动。
服用胆囊收缩素(CCK)或胰高血糖素样肽1(GLP-1)受体激动剂Exendin-4(Ex-4)可减少食物摄入量。文献研究结果表明,CCK主要作为饱腹感信号减少摄入量,而GLP-1可能在饱腹感和与奖赏相关的摄入信号中都发挥作用。被人类描述为 "甜 "和 "肥 "的化合物都是适口的,但它们通过不同的传导途径发出信号。在这里,实验人员通过简短的舔食过程,测量了限制进食的雄性大鼠对Ex-4(实验前3小时)、CCK(实验前30分钟)或二者联合作用的非条件舔食反应。目前的实验设计是在30分钟的单次测试过程中,在10秒钟的试验中测量大鼠对水以及不同浓度的蔗糖(0.03、0.1和0.5 M)和内脂质(0.2%、2%和20%)的舔舐反应。这种设计最大程度地减少了后estive影响。与注射生理盐水的对照组相比,CCK(1.0、3.0或6.0 μg/kg)不会改变对蔗糖或内脂的舔舐反应。注射了 3.0 µg/kg(而非 1.0 µg/kg Ex-4)的大鼠开始的试验次数以及对蔗糖和内脂的舔舐反应均有所减少。与单独注射 Ex-4 相比,补充 CCK 不会改变舔食反应或开始的试验。这些研究结果支持GLP-1而非CCK在口腔对适口刺激的反应中发挥作用。此外,还观察到Ex-4诱导的蔗糖和内脂(分别代表 "甜味 "和 "脂肪")反应降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemical Senses
Chemical Senses 医学-行为科学
CiteScore
8.60
自引率
2.90%
发文量
25
审稿时长
1 months
期刊介绍: Chemical Senses publishes original research and review papers on all aspects of chemoreception in both humans and animals. An important part of the journal''s coverage is devoted to techniques and the development and application of new methods for investigating chemoreception and chemosensory structures.
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