Mitochondrial DNA content as a biomarker for oral carcinogenesis: correlation with clinicopathologic parameters.

IF 1.1 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Minerva dental and oral science Pub Date : 2023-10-01 Epub Date: 2023-04-17 DOI:10.23736/S2724-6329.23.04756-3
Reema Raina, Devi C Shetty, Nighat Nasreen, Shukla DAS, Aashka Sethi, Atul Chikara, Gargi Rai, Anshuman Kumar, Sonam Tulsyan, Sandeep Sisodiya, Showket Hussain
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引用次数: 0

Abstract

Background: Mitochondrial genome (mtDNA) exhibits greater vulnerability to mutations and/or copy number variations than nuclear counterpart (nDNA) in both normal and cancer cells due to oxidative stress generated by inflammation, viral infections, physical, mechanical, and chemical load. The study was designed to evaluate the mtDNA content in oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC). Various parameters were analyzed including its variation with human papillomavirus (HPV) during oral carcinogenesis.

Methods: The present cross-sectional study comprised of two hundred patients (100 OPMDs and 100 OSCCs) and 100 healthy controls. PCR amplifications were done for mtDNA content and HPV in OPMDs and OSCC using real-time and conventional PCR respectively.

Results: The relative mtDNA content was assessed quantitatively and it was observed that mtDNA was greater in OSCC (7.60±0.94) followed by OPMDs (5.93±0.92) and controls (5.37±0.95). It showed a positive linear correlation with habits and increasing histopathological grades. Total HPV-positive study groups showed higher mtDNA content (7.06±1.64) than HPV-negative counterparts (6.21±1.29).

Conclusions: An elevated mutant mtDNA may be attributed to increased free radicals and selective cell clonal proliferation in test groups. Moreover, sustained HPV infection enhances tumorigenesis through mitochondria mediated apoptosis. Since, mtDNA content is directly linked to oxidative DNA damage, these quantifications might serve as a surrogate measure for invasiveness in dysplastic lesions and typify their malignant potential.

线粒体DNA含量作为口腔癌变的生物标志物:与临床病理参数的相关性。
背景:在正常和癌症细胞中,由于炎症、病毒感染、物理、机械和化学负荷产生的氧化应激,线粒体基因组(mtDNA)比核对应物(nDNA)更容易受到突变和/或拷贝数变化的影响。本研究旨在评估口腔潜在恶性疾病(OPMD)和口腔鳞状细胞癌(OSCC)中mtDNA的含量。分析了各种参数,包括其在口腔癌变过程中与人乳头瘤病毒(HPV)的变化。方法:本横断面研究包括200名患者(100名OPMD和100名OSCC)和100名健康对照。分别使用实时和常规PCR对OPMD和OSCC中的mtDNA含量和HPV进行PCR扩增。结果:定量评估了线粒体DNA的相对含量,发现OSCC的线粒体DNA含量较高(7.60±0.94),其次是OPMD(5.93±0.92)和对照组(5.37±0.95)。总的HPV阳性研究组的mtDNA含量(7.06±1.64)高于HPV阴性研究组的(6.21±1.29)。结论:突变mtDNA的升高可能归因于试验组中自由基的增加和选择性细胞克隆增殖。此外,持续的HPV感染通过线粒体介导的细胞凋亡来增强肿瘤的发生。由于mtDNA含量与DNA氧化损伤直接相关,这些定量可能作为衡量发育异常病变侵袭性的替代指标,并代表其恶性潜能。
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来源期刊
Minerva dental and oral science
Minerva dental and oral science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
2.50
自引率
5.00%
发文量
61
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