Solutions Containing a Statherin-Derived Peptide Reduce Enamel Erosion in vitro.

IF 2.9 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Caries Research Pub Date : 2023-01-01 DOI:10.1159/000529016

Fabiana Navas Reis, Monique Malta Francese, Natara Dias Gomes da Silva, Vinícius Taioqui Pelá, João Victor Frazão Câmara, Juliana Sanches Trevizol, Heitor Marques Honorio, Edson Crusca, Reinaldo Marchetto, Marília Afonso Rabelo Buzalaf

{"title":"Solutions Containing a Statherin-Derived Peptide Reduce Enamel Erosion in vitro.","authors":"Fabiana Navas Reis, Monique Malta Francese, Natara Dias Gomes da Silva, Vinícius Taioqui Pelá, João Victor Frazão Câmara, Juliana Sanches Trevizol, Heitor Marques Honorio, Edson Crusca, Reinaldo Marchetto, Marília Afonso Rabelo Buzalaf","doi":"10.1159/000529016","DOIUrl":null,"url":null,"abstract":"<p><p>The effect of solutions containing a statherin-derived peptide (Stn15pSpS) on the protection against enamel erosion in vitro was evaluated. Bovine enamel specimens were divided into 4 groups (n = 15/group): (1) deionized water (negative control), (2) Elmex Erosion Protection™ (positive control), (3) 1.88 × 10-5 M Stn15pSpS, and (4) 3.76 × 10-5 M Stn15pSpS. The solutions were applied on the specimens for 1 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH-cycling protocol 4 times/day, for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The solutions were applied again during pH-cycling, 2 times/day for 1 min after the first and last erosive challenges. Enamel loss (μm) was assessed by contact profilometry. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05). The best protection against erosion was conferred by Elmex Erosion Protection that significantly differed from all the other treatments, followed by the solutions containing Stn15pSpS, regardless of the concentration. However, 3.76 × 10-5 M Stn15pSpS did not differ from the negative control. The solution containing the lower concentration of Stn15pSpS protected against erosion in vitro, which should be confirmed using protocols that more closely resemble the clinical condition.</p>","PeriodicalId":9620,"journal":{"name":"Caries Research","volume":"57 1","pages":"52-58"},"PeriodicalIF":2.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Caries Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000529016","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 2

Abstract

The effect of solutions containing a statherin-derived peptide (Stn15pSpS) on the protection against enamel erosion in vitro was evaluated. Bovine enamel specimens were divided into 4 groups (n = 15/group): (1) deionized water (negative control), (2) Elmex Erosion Protection™ (positive control), (3) 1.88 × 10-5 M Stn15pSpS, and (4) 3.76 × 10-5 M Stn15pSpS. The solutions were applied on the specimens for 1 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH-cycling protocol 4 times/day, for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The solutions were applied again during pH-cycling, 2 times/day for 1 min after the first and last erosive challenges. Enamel loss (μm) was assessed by contact profilometry. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05). The best protection against erosion was conferred by Elmex Erosion Protection that significantly differed from all the other treatments, followed by the solutions containing Stn15pSpS, regardless of the concentration. However, 3.76 × 10-5 M Stn15pSpS did not differ from the negative control. The solution containing the lower concentration of Stn15pSpS protected against erosion in vitro, which should be confirmed using protocols that more closely resemble the clinical condition.

查看原文本刊更多论文

含有statherin衍生肽的溶液可减少体外牙釉质侵蚀。

研究了含石蜡素衍生肽(stn15psp)的溶液对体外牙釉质侵蚀的保护作用。牛牙釉质标本分为4组(n = 15/组):(1)去离子水(阴性对照)，(2)Elmex Erosion Protection™(阳性对照)，(3)1.88 × 10-5 M Stn15pSpS， (4) 3.76 × 10-5 M Stn15pSpS。溶液作用于标本1分钟。从3个供体收集刺激唾液，用于在标本上形成2小时获得的膜。然后，将标本提交到侵蚀pH循环方案中，每天4次，共7天(0.01 M HCl pH 2.0/45 s，人工唾液/2 h，人工唾液过夜)。在ph循环期间再次施用这些溶液，在第一次和最后一次侵蚀后，每天2次，持续1分钟。牙釉质损失(μm)采用接触轮廓法测定。数据采用Kruskal-Wallis和Dunn’s检验分析(p < 0.05)。与其他处理相比，Elmex erosion protection提供了最好的抗侵蚀保护，其次是含有stn15psp的溶液，无论浓度如何。然而，3.76 × 10-5 M stn15psp与阴性对照没有差异。含有较低浓度stn15psp的溶液在体外可以防止腐蚀，这一点需要使用更接近临床条件的方案来证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Caries Research 医学-牙科与口腔外科

CiteScore

6.30

自引率

7.10%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Caries Research'' publishes epidemiological, clinical and laboratory studies in dental caries, erosion and related dental diseases. Some studies build on the considerable advances already made in caries prevention, e.g. through fluoride application. Some aim to improve understanding of the increasingly important problem of dental erosion and the associated tooth wear process. Others monitor the changing pattern of caries in different populations, explore improved methods of diagnosis or evaluate methods of prevention or treatment. The broad coverage of current research has given the journal an international reputation as an indispensable source for both basic scientists and clinicians engaged in understanding, investigating and preventing dental disease.