Bacteriophage T4 as a nanovehicle for delivery of genes and therapeutics into human cells

IF 5.7 2区 医学 Q1 VIROLOGY
Venigalla B Rao, Jingen Zhu
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引用次数: 4

Abstract

The ability to deliver therapeutic genes and biomolecules into a human cell and restore a defective function has been the holy grail of medicine. Adeno-associated viruses and lentiviruses have been extensively used as delivery vehicles, but their capacity is limited to one (or two) gene(s). Bacteriophages are emerging as novel vehicles for gene therapy. The large 120 × 86-nm T4 capsid allows engineering of both its surface and its interior to incorporate combinations of DNAs, RNAs, proteins, and their complexes. In vitro assembly using purified components allows customization for various applications and for individualized therapies. Its large capacity, cell-targeting capability, safety, and inexpensive manufacturing could open unprecedented new possibilities for gene, cancer, and stem cell therapies. However, efficient entry into primary human cells and intracellular trafficking are significant barriers that must be overcome by gene engineering and evolution in order to translate phage-delivery technology from bench to bedside.

T4噬菌体作为一种纳米载体,将基因和治疗药物输送到人体细胞中
将治疗基因和生物分子输送到人体细胞中并恢复有缺陷的功能一直是医学界的圣杯。腺相关病毒和慢病毒已被广泛用作运载工具,但它们的能力仅限于一个(或两个)基因。噬菌体正在成为基因治疗的新型载体。巨大的120 × 86纳米T4衣壳允许对其表面和内部进行工程设计,以结合dna, rna,蛋白质及其复合物的组合。体外组装使用纯化组件允许定制各种应用和个性化治疗。它的大容量,细胞靶向能力,安全性和廉价的制造可以为基因,癌症和干细胞治疗开辟前所未有的新可能性。然而,有效进入原代人类细胞和细胞内运输是基因工程和进化必须克服的重大障碍,以便将噬菌体传递技术从实验室转化为临床。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
11.80
自引率
5.10%
发文量
76
审稿时长
83 days
期刊介绍: Current Opinion in Virology (COVIRO) is a systematic review journal that aims to provide specialists with a unique and educational platform to keep up to date with the expanding volume of information published in the field of virology. It publishes 6 issues per year covering the following 11 sections, each of which is reviewed once a year: Emerging viruses: interspecies transmission; Viral immunology; Viral pathogenesis; Preventive and therapeutic vaccines; Antiviral strategies; Virus structure and expression; Animal models for viral diseases; Engineering for viral resistance; Viruses and cancer; Virus vector interactions. There is also a section that changes every year to reflect hot topics in the field.
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