Effects of the SPI/lncRNA NEAT1 Axis on Functions of Trophoblast and Decidual Cells in Patients with Recurrent Miscarriage.

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Fei Tian, Yuan Zhang, Jie Li, Zhaoping Chu, Junqin Zhang, Hua Han, Ligang Jia
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引用次数: 0

Abstract

Recurrent miscarriage (RM) is a frustrating and complex pregnancy disorder and long noncoding RNAs (lncRNAs) modulate susceptibility to RM. This study expounded on the role of specificity protein 1 (SP1) in functions of chorionic trophoblast and decidual cells via regulating lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1). Chorionic villus tissues and decidual tissues of RM patients and normal pregnant women were collected. Real-time quantitative polymerase chain reaction and Western blotting revealed that SP1 and NEAT1 were downregulated in trophoblast and decidual tissues of RM patients, and the Pearson correlation analysis detected that they were positively correlated in expression level. Chorionic trophoblast and decidual cells of RM patients were isolated and intervened by vectors over-expressing SP1 or NEAT1 siRNAs. Thereafter, the cell counting kit-8, Transwell, flow cytometry assays detected that SP1 overexpression accelerated trophoblast cell proliferation, invasion, and migration, meanwhile, enhancing decidual cell proliferation while repressed apoptosis. Next, the dual-luciferase and Chromatin immunoprecipitation assays showed that SP1 bound to the NEAT1 promoter region and further activated NEAT1 transcription. Silencing NEAT1 reversed the efforts of SP1 overexpression on the functions of trophoblast and decidual cells. Overall, SP1 activated NEAT1 transcription, accelerating trophoblast cell proliferation, invasion, and migration and mitigating decidual cell apoptosis.

SPI/lncRNA NEAT1轴对复发性流产患者滋养细胞和蜕膜细胞功能的影响。
复发性流产(RM)是一种令人沮丧和复杂的妊娠疾病,长链非编码rna (lncRNAs)调节了对RM的易感性。本研究阐述了特异性蛋白1 (SP1)通过调控lncRNA核副斑组装转录本1 (NEAT1)在绒毛膜滋养细胞和蜕膜细胞功能中的作用。收集RM患者和正常孕妇的绒毛膜绒毛组织和蜕膜组织。实时定量聚合酶链反应和Western blotting结果显示,SP1和NEAT1在RM患者的滋养细胞和蜕膜组织中表达下调,Pearson相关分析发现两者表达水平呈正相关。分离RM患者的绒毛膜滋养细胞和蜕膜细胞,用过表达SP1或NEAT1 sirna的载体进行干预。随后,细胞计数试剂盒-8、Transwell、流式细胞术检测发现SP1过表达加速了滋养细胞的增殖、侵袭和迁移,同时增强了蜕膜细胞的增殖,抑制了凋亡。接下来,双荧光素酶和染色质免疫沉淀实验显示SP1结合到NEAT1启动子区域并进一步激活NEAT1转录。沉默NEAT1可逆转SP1过表达对滋养细胞和蜕细胞功能的影响。总的来说,SP1激活了NEAT1的转录,加速了滋养细胞的增殖、侵袭和迁移,减轻了蜕膜细胞的凋亡。
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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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