MIR503HG Overexpression Inhibits the Malignant Behaviors of Osteosarcoma Cells by Sponging miR-103a-3p.

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Enhui Li, Shoubin Zhong, Guikai Ma, Qian Wang, Yanfang Gao
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引用次数: 1

Abstract

Osteosarcoma (OS) is the most representative primary bone tumour in children and teenagers. This study explored the regulatory effects of long noncoding RNA MIR503HG (MIR503HG) on the biological functions of OS cells, and further investigated the potential mechanism of MIR503HG function exertion by analyzing the microRNA-103a-3p (miR-103a-3p) in OS cells and tissues. The expression of MIR503HG was examined using reverse transcription-quantitative PCR. OS cell proliferation was assessed by CCK-8 assay. Transwell assay was used to evaluate the migration and invasion of OS cells. The interaction between MIR503HG and miR-103a-3p was detected using the Dual-luciferase reporter assay. Forty-six paired OS tissues were collected, and the expression and correlation of MIR503HG and miR-103a-3p were evaluated. The expression of MIR503HG were significantly decreased in both OS cells and tissues. Over-expression of MIR503HG inhibited OS cell proliferation, migration and invasion. miR-103a-3p was directly targeted by MIR503HG in OS cells, and mediated the inhibitory effects of MIR503HG on OS cell malignant behaviors. miR-103a-3p expression was upregulated in OS tissues, which was negatively correlated with MIR503HG expression levels. The expression of MIR503HG was associated with OS patients' tumor size, differentiation, distant metastasis and clinical stage. Decreased MIR503HG in OS tissues and cell lines served as a tumor suppressor by inhibiting OS cell malignant behaviors through sponging miR-103a-3p. The findings of this study may provide evidence for the development of novel therapeutic targets of OS.

MIR503HG过表达通过海绵miR-103a-3p抑制骨肉瘤细胞的恶性行为。
骨肉瘤(Osteosarcoma, OS)是儿童和青少年最具代表性的原发性骨肿瘤。本研究探讨长链非编码RNA MIR503HG (MIR503HG)对OS细胞生物学功能的调控作用,并通过分析OS细胞和组织中的microRNA-103a-3p (miR-103a-3p)进一步探讨MIR503HG功能发挥的潜在机制。采用逆转录-定量PCR检测MIR503HG的表达。CCK-8法检测OS细胞增殖情况。Transwell法观察OS细胞的迁移和侵袭情况。采用双荧光素酶报告基因法检测MIR503HG和miR-103a-3p之间的相互作用。收集46对OS组织,评估MIR503HG和miR-103a-3p的表达及相关性。MIR503HG在OS细胞和组织中的表达均显著降低。MIR503HG过表达抑制OS细胞增殖、迁移和侵袭。miR-103a-3p在OS细胞中被MIR503HG直接靶向,介导MIR503HG对OS细胞恶性行为的抑制作用。miR-103a-3p在OS组织中表达上调,与MIR503HG表达水平呈负相关。MIR503HG的表达与OS患者肿瘤大小、分化、远处转移及临床分期有关。在OS组织和细胞系中MIR503HG的降低通过海绵化miR-103a-3p抑制OS细胞的恶性行为,起到抑瘤作用。本研究结果可能为开发新的OS治疗靶点提供依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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